| Texto completo | |
| Autor(es): |
de Freitas, Lucas Victor Pereira
;
Alponti, Ana Luisa Bigliassi
;
Campanharo, Sarah Chagas
;
Damaceno, Marina Alves
;
da Silva, Agnaldo Fernando Baldo
;
Souza, Marilia Cristina de Oliveira
;
Assane, Inacio Mateus
;
Pilarski, Fabiana
;
Sasanya, James Jacob
;
Paschoal, Jonas Augusto Rizzato
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Food Chemistry; v. 496, p. 13-pg., 2025-12-25. |
| Resumo | |
Sample preparation is a crucial and challenging step for determining veterinary drug residues (VDRs) in animal products. This study aimed to develop two novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for the determination of levamisole in fish fillet (muscle plus skin in natural proportion): Low-Temperature Partition Extraction (LTPE) and a combination of LTPE with Dispersive Liquid-Liquid Microextraction (DLLME). Optimal extraction conditions were established through multivariate optimization. Both methods were validated, demonstrating selectivity, precision (CV < 20 %), accuracy (recoveries 98 %-111 %), low matrix effects, and linearity in the ranges of 2-200 and 0.2-20 mu g kg(-1) for LTPE and LTPE-DLLME, respectively. The LTPE method was successfully applied in the analysis of real samples to evaluate levamisole residues in fish. To the best of our knowledge, this is the first study to combine LTPE and DLLME, representing a promising advance in sensitive and selective methods for routine monitoring of levamisole and potentially other VDRs in food-producing animals. (AU) | |
| Processo FAPESP: | 21/03294-1 - Estudos para o uso de levamisol na Aquicultura: métodos analíticos; incorporação na ração; depleção residual; e estimativa de período de carência |
| Beneficiário: | Lucas Victor Pereira de Freitas |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 21/08152-0 - Desafio do uso de medicamentos na piscicultura no contexto One Health: avaliação da eficácia, segurança e análise de risco |
| Beneficiário: | Jonas Augusto Rizzato Paschoal |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 21/11996-6 - Avaliação de procedimentos de incorporação do levamisol na ração de peixes |
| Beneficiário: | Ana Luísa Bigliassi Alponti |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |