Autor(es)

Kamezawa, Joao G. Veneziani ; Kato, Lilian Seiko ; Fonseca, Elisania Kelly Barbosa ; Arruda, Marco Aurelio Zezzi

• Tipo de documento: Artigo Científico
• Fonte: BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY; v. 12, n. 49, p. 8-pg., 2025-10-01.

Resumo

Tissue and cell culture techniques have become pivotal in plant biotechnology, particularly for the improvement and mass propagation of economically important species such as soybean (Glycine max). Within this context, somatic embryogenesis has emerged as a powerful tool. Micronutrients like Fe, Mn, Mo, and Zn play essential roles in cellular metabolism and can serve as indicators of tissue growth and development. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) is widely recognized for its sensitivity in multielement analysis at trace levels; however, sample preparation remains a critical and often resource-intensive step. This study aims to develop and compare three distinct sample preparation methods for the determination of micronutrients in soybean calli using ICP-MS: (1) conventional acid decomposition, (2) acid extraction, and (3) acidless decomposition. The results indicated that all three methods provided statistically equivalent concentrations for all evaluated elements (p >= 0.05), with values ranging from 0.54 +/- 0.08 mu g g-1 for Mo to 14 +/- 1 mu g g-1 for Mn. Additionally, the residual chemical content (RCC) in the proposed methods was below the detection limit of 8 mg L-1. Sustainability assessment using the AGREEprep tool highlighted acidless decomposition as the most environmentally friendly method, primarily due to its use of greener reagents and higher sample throughput. These findings suggest that acidless decomposition presents a viable and sustainable alternative for micronutrient analysis in plant tissue culture applications. (AU)

• Processo FAPESP: 21/15218-8 - Nanometalômica aplicada ao cultivo da soja
• Beneficiário: Lilian Seiko Kato
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 22/13166-3 - Nanoionômica aplicada para cultivo de soja
• Beneficiário: João Gabriel Veneziani Kamezawa
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/24445-8 - EMU concedido no processo 2018/25207-0: espectrômetro de massas com plasma indutivamente acoplado
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 18/25207-0 - Nanometalômica aplicada ao cultivo da soja para estudar os efeitos das nanopartículas relacionadas a metal
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático