| Texto completo | |
| Autor(es): |
Dos Santos, Lucas Nascimento
;
Cavalcante, Lidia Do Nascimento
;
Dutt, Manjul
;
De Souza, Alessandra Alves
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF APPLIED GENETICS; v. N/A, p. 13-pg., 2026-01-08. |
| Resumo | |
The Sieve Element Occlusion (SEO) proteins are subunits that assemble into structural filamentous phloem proteins, commonly referred to as P-proteins. These proteins in Arabidopsis play a structural role and contribute to plant defense by reversible sieve plate (SP) sealing. This mechanism could be particularly important also in Citrus trees affected by Huanglongbing (HLB), as SEO genes are highly induced in susceptible species C. sinensis upon Candidatus Liberibacter asiaticus (CLas) colonization. Given the limited information on this gene family in Citrus, we analyzed 27 SEO genes within the Rutaceae family, with a focus on genera closely related to Citrus, to better understand their potential roles in HLB tolerance. Genomic sequences revealed conserved exon-intron structures similar to Arabidopsis thaliana, while promoter regions contained a higher number of light-responsive Cis-elements, along with elements associated with growth, development, stress responses, and phloem-specific gene expression. Subcellular localization identified the cytoplasm as the primary site, with additional predictions for the plasma membrane and mitochondria. Phylogenetic analysis categorized SEO proteins into five subgroups, refining their classification in Citrus. Lately, protein interaction networks indicated strong connections with proteins involved in coordinated immune responses. These findings improve the understanding of SEO protein dynamics and evolutionary conservation, highlighting their role in phloem biology. Further investigation of these SEO genes and their promoters in the plant response to HLB could help identify specific targets for developing disease-tolerant Citrus varieties through genetic engineering. (AU) | |
| Processo FAPESP: | 23/05118-1 - Potencial das plantas de citros geneticamente modificadas supexpressando o gene rpfF no controle de doenças bacterianas de citros. |
| Beneficiário: | Alessandra Alves de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/07045-3 - Estratégias biotecnológicas e genômicas para qualidade, produtividade e manejo sustentável de citros, café e cana-de-açúcar no estado de São Paulo |
| Beneficiário: | Mariângela Cristofani-Yaly |
| Modalidade de apoio: | Auxílio à Pesquisa - Núcleos de Pesquisa Orientada a Problemas em São Paulo |
| Processo FAPESP: | 23/10399-0 - Aprimorando a transformação da laranja doce por meio de culturas de células embriogênicas usando a edição mediada por CRISPR-Cas9 do gene Sieve Element Occlusion |
| Beneficiário: | Lucas Nascimento dos Santos |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Mestrado |
| Processo FAPESP: | 22/12483-5 - Sistema CRISPR/Cas9 na edição do gene Sieve Element Occlusion c (SEOc), visando obtenção de plantas de citros tolerantes ao Huanglongbing (HLB) |
| Beneficiário: | Lucas Nascimento dos Santos |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |