Validation of a gas chromatographic method to quantify sesquiterpenes in copaiba oils

Autor(es)

Sousa, Joao Paulo B. ; Brancalion, Ana P. S. ; Souza, Ariana B. ^[1] ; Turatti, Izabel C. C. ^[2] ; Ambrosio, Sergio R. ^[1] ; Furtado, Niege A. J. C. ; Lopes, Norberto P. ^[2] ; Bastos, Jairo K. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Franca, Nucleo Pesquisa Ciencias Exatas & Tecnol, BR-14404600 Franca, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Lab Quim Organ, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, Lab Farmacognosia, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmaceutical and Biomedical Analysis; v. 54, n. 4, p. 653-659, MAR 25 2011.
• Citações Web of Science: 34

Resumo

Copaifera species (Leguminoseae) are popularly known as ``copaiba{''} or ``copaiva{''}. The oleoresins obtained from the trunk of these species have been extensively used in folk medicine and are commercialized in Brazil as crude oil and in several pharmaceutical and cosmetic products. This work reports a complete validated method for the quantification of beta-caryophyllene, alpha-copaene, and alpha-humulene in distinct copaiba oleoresins available commercially. Thus, essential oil samples (100 mu L) were dissolved in 20 mL of hexanes containing internal standard (1,2,4,5-tetramethylbenzene, 3.0 mM) in a 25 mL glass flask. A 1 mu L aliquot was injected into the GC-FID system. A fused-silica capillary column HP-5, coated with 5% phenylmethylsiloxane was used for this study. The developed method gave a good detection response with linearity in the range of 0.10-18.74 mM. Limits of detection and quantitation variety ranged between 0.003 and 0.091 mM. beta-Caryophyllene, alpha-copaene, and alpha-humulene were recovered in a range from 74.71% to 88.31%, displaying RSD lower than 10% and relative errors between -11.69% and -25.30%. Therefore, this method could be considered as an analytical tool for the quality control of different Copaifera oil samples and its products in both cosmetic and pharmaceutical companies. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 06/59893-0 - Desenvolvimento de metodologia analítica e estudo fitoquímico do extrato hidroalcoólico e da fração volátil das folhas e ramos de Copaifera langsdorffii (Leguminosae: Caesalpinioideae)
• Beneficiário: João Paulo Barreto de Sousa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/57775-6 - Estudo fitoquímico, desenvolvimento de método analítico e investigação das propriedades antilitiásicas das partes aéreas de Copaifera langsdorffii
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Regular