| Texto completo | |
| Autor(es): |
Bomediano, Mateus P.
;
Plivelic, Tomas S.
;
Pedersen, Jan Skov
;
Galantini, Luciano
;
Schillen, Karin
;
De Oliveira, Marcelo G.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS; v. 730, p. 15-pg., 2025-02-05. |
| Resumo | |
We report the first incorporation of S-nitroso-N-acetylpenicillamine (SNAP) into micellar systems of the poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer Pluronic (R) F127 for nitric oxide (NO) delivery and show how sodium taurodeoxycholate (NaTDC) modulates F127 micellization and gelation. Using differential scanning calorimetry, small-angle X-ray scattering, rheology, dynamic light scattering, UV-vis spectroscopy, and chemiluminescence NO analysis, we identify two NaTDC-dependent regimes. At low NaTDC/F127 ratios (MR <= similar to 1.2), NaTDC associates with F127 micelles, lowering the critical micellization temperature and modestly compacting the PPO cores. At higher ratios (MR >= similar to 3.8), micellization becomes less cooperative, small bile salt-rich complexes form, and at 25 wt% F127, the gel nanostructure transitions from face-centered cubic phase to body-centered cubic phase and eventually loses long-range order (MR similar to 6.2). SNAP load does not alter micellization, locates at the core-corona interface, and shows enhanced solubility that depends more on F127 concentration than on NaTDC. Thermal NO release from SNAP (25 degrees C, dark) follows second-order kinetics, slowing as F127 concentration increases (1-25 wt%), resulting in prolonged NO release. These findings contribute to advancing the understanding of the behavior of PEO-PPO-PEO/NaTDC micellar systems, by demonstrating how NaTDC regulates micellization and gelation. Moreover, the finding that F127 concentration controls SNAP stability and NO-release kinetics, makes the F127/NaTDC system a potential modular platform for sustained and localized NO delivery. (AU) | |
| Processo FAPESP: | 24/05080-7 - Incorporação de sais biliares de colil-nitroso-cisteína em hidrogéis de Pluronic: impacto no empacotamento micelar |
| Beneficiário: | Mateus Peres Bomediano |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto |
| Processo FAPESP: | 22/14645-2 - Hidrogéis e próteses vasculares liberadores de óxido nítrico para aplicações cardiovasculares |
| Beneficiário: | Marcelo Ganzarolli de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 22/13352-1 - Próteses vasculares de hidrogéis micelares doadores de óxido nítrico |
| Beneficiário: | Mateus Peres Bomediano |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |