| Texto completo | |
| Autor(es): |
Guzman-Moreno, Cesar W.
;
Cardinali-Rezende, Juliana
;
Di Genova, Alex
;
Ferrarini, Mariana
;
Sagot, Marie-France
;
Rodrigues, Kelli L.
;
Silva, Luiziana F.
;
Taciro, Marilda K.
;
Gomez, Jose Gregorio C.
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Biological Macromolecules; v. 337, p. 16-pg., 2026-01-01. |
| Resumo | |
Halomonas sp. HG01, a moderate halophilic bacterium isolated from a northern Peru salt mine, is promising as a polyhydroxyalkanoate (PHA) producer. Experimental data and genome analysis were used to evaluate its capabilities. Shaken flask experiments revealed that HG01 can accumulate 70-86 wt% poly(3-hydroxybutyrate) [P (3HB)] from various carbon sources, including glucose, sucrose, and fructose. In a fed-batch bioreactor, it achieved a cell dry weight (CDW) of 12.2 g/L with 63 % P(3HB) content after 72 hours. The PHA synthase enzyme exhibited substrate specificity for C4 to C5 compounds. HG01 strain also produced poly(3-hydrox-ybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] from propionic and valeric acids, with a maximum 3HV content of 25.53 mol% monomers into the polymer (69.40 %wt) when valeric acid was used. The complete bacterial 3.66 Mbp genome sequence revealed metabolic pathways for carbohydrate and fatty acid catabolism, PHA biosynthesis, and stress tolerance factor. This genetic information enhances our understanding of PHA synthesis and supports the development of metabolic engineering strategies, positioning Halomonas sp. HG01 as a promising candidate for biotechnological applications. (AU) | |
| Processo FAPESP: | 14/08061-1 - Reconstrução da rede metabólica em escala genômica e melhoramento da produção de polihidroxialcanoatos em Pseudomonas (LFM046) |
| Beneficiário: | Juliana Cardinali Rezende |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 16/24290-6 - Análise do perfil transcricional de Pseudomonas sp. LFM046 e seus recombinantes produzindo biopolímeros / polihidroxialcanoatos a partir de glicose baseado em DNA Microarray e RNASeq. |
| Beneficiário: | Juliana Cardinali Rezende |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |
| Processo FAPESP: | 10/51692-1 - Engenharia metabólica aplicada à produção de biopolímero |
| Beneficiário: | Marilda Keico Taciro |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores |