Molecular interactions and structure of a supramolecular arrangement of glucose oxidase and palladium nanoparticles

Autor(es)

Pereira, Andressa R. ^[1] ; Iost, Rodrigo M. ^[1] ; Martins, Marccus V. A. ^[1] ; Yokomizo, Cesar H. ^[2] ; da Silva, Welter C. ^[3] ; Nantes, Iseli L. ^[1] ; Crespilho, Frank N. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre - Brazil

^[2] Univ Fed Sao Paulo, Dept Biol Mol, BR-04044020 Sao Paulo - Brazil

^[3] Univ Fed Piaui, Ctr Ciencias Nat, Dept Quim, BR-64049550 Teresina, PI - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Physical Chemistry Chemical Physics; v. 13, n. 26, p. 12155-12162, 2011.
• Citações Web of Science: 12

Resumo

This paper presents studies about the molecular interactions and redox processes involved in the formation of palladium nanoparticles associated to glucose oxidase (GOx-PdNPs) in a supramolecular arrangement. The synthesis occurs in two steps, the Pd reduction and the formation of the 80 nm sized supramolecular aggregates containing multiples units of GOx associated to 3.5 nm sized PdNPs. During synthesis, GOx molecules interact with Pd salt leading to metal ion and FAD reduction probably via the thiol group of the cysteine 521 residue. For the growing of PdNPs, formic acid was necessary as a co-adjuvant reducing agent. Besides the contribution for the redox processes, GOx is also necessary for the NP stability preventing the formation of precipitates resulted from uncontrolled growing of NPs Cyclic voltammetry of the GOx-PdNPs demonstrated electroactivity of the bionanocomposite immobilized on ITO (indium-tin oxide) electrode surface and also the NP is partially blocked due to strong interaction GOx and the surface of PdNPs. Vibrational spectroscopy (FTIR) showed that significant structural changes occurred in GOx after the association to PdNP. These mechanistics and structural studies can contribute for modulation of bionanocomposites properties. (AU)

• Processo FAPESP: 09/15558-1 - Interação entre biomoléculas e sistemas celulares com nanoestruturas 0D, 1D e 2D utilizando métodos eletroquímicos
• Beneficiário: Frank Nelson Crespilho
• Modalidade de apoio: Auxílio à Pesquisa - Regular