| Texto completo | |
| Autor(es): |
Avanzi, R. D. T.
[1]
;
Cavarsan, C. F.
[1]
;
Santos, Jr., J. G.
[2]
;
Hamani, C.
[1]
;
Mello, L. E.
[1]
;
Covolan, L.
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, Dept Fisiol, BR-04023062 Sao Paulo - Brazil
[2] Fac Ciencias Med Santa Casa de Sao Paulo, BR-01221020 Sao Paulo - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Neuroscience; v. 170, n. 3, p. 687-691, OCT 27 2010. |
| Citações Web of Science: | 14 |
| Resumo | |
Epilepsy is known to influence hippocampal dentate granule cell (DGC) layer neurogenesis. In young adult rats, status epilepticus (SE) increases the number DGC newly borne cells and basal dendrites (BD), which persist at long-term. In contrast, little is known on whether these phenomena occur in elderly epileptic animals. In the present study, we compare DGC proliferation and the incidence of BD in young and aged pilocarpine-treated rats. Three epileptic groups were considered: Young animals given pilocarpine at 3 months of age. Aged animals treated with pilocarpine at 3 months of age that were sacrificed at 17-20 months. Aged animals that had pilocarpine and developed SE at 20 months, being sacrificed 2 months later. Nine days prior to sacrifice, animals underwent swimming sessions in the Morris water maze as a protocol for the development of hippocampal neurogenesis. We found a higher incidence of newly born DGC cells in young as compared to aged epileptic animals (P<0.001). This later group however, was not homogeneous. While a significant increase in DGC neurogenesis was observed when aged animals with long lasting epilepsy were compared to non-epileptic controls (P<0.01), this has not been recorded in aged animals that had epilepsy for only 2 months (P>0.05). When the number of DGC containing BD was considered, a significantly higher incidence was observed in young as compared to aged epileptic rats (P=0.001). Animals in this later group virtually lacked BD in newly formed dentate gyrus (DG) cells. Based on these results we conclude that plastic changes during epileptogenesis and the development of a pathological substrate in young animals is associated with DGC proliferation and the emergence of BD. As aging occurs, DGC neurogenesis can still be induced in rats with a long-term history of epilepsy but the emergence of BD is markedly reduced. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 06/60256-5 - Avaliação da relação entre aprendizagem e memória, neurogênese e envelhecimento, em animais epilépticos crônicos utilizando o modelo da pilocarpina |
| Beneficiário: | Luciene Covolan |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |