Molecular characterization of Cryptosporidium spp. from fecal samples of birds kept in captivity in Brazil

Autor(es)

Nakamura, Alex Akira ^[1] ; Simoes, Daniel Castendo ^[2] ; Antunes, Romulo Godik ^[2] ; da Silva, Deuvania Carvalho ^[2] ; Meireles, Marcelo Vasconcelos ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, FMVZ, BR-09500900 Sao Paulo - Brazil

^[2] Sao Paulo State Univ, Curso Med Vet, Dept Clin Cirurgia & Reprod Anim, BR-16050680 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Parasitology; v. 166, n. 1-2, p. 47-51, DEC 3 2009.
• Citações Web of Science: 43

Resumo

The aim of this study was to determine the prevalence of Cryptosporidium species and genotypes in birds kept in captivity in Brazil. A total of 966 samples from 18 families of birds was collected and stored in 5% potassium dichromate solution at 4 degrees C until processing. Oocysts were purified in Sheather sugar solution following extraction of genomic DNA. Molecular analyses were performed using nested-PCR for amplification of fragments of the 18S subunit of rRNA gene and of the actin gene. Amplification of Cryptosporidium DNA fragments was obtained in 47 (4.86%) samples. Sequencing of amplified fragments and phylogenetic analyses allowed the identification of Cryptosporidium baileyi in a black vulture (Coragyps atratus), a domestic chicken (Gallus gallus domesticus) and a saffron finch (Sicalis flaveola); Cryptosporidium galli in canaries (Serinus canaria), a cockatiel (Nymphicus hollandicus) and lesser seed-finches (Oryzoborus angolensis); Cryptosporidium meleagridis in a domestic chicken (G. g. domesticus); Cryptosporidium parvum in a cockatiel (N. hollandicus); Cryptosporidium avian genotype I in a canary (S. canaria) and an Indian peafowl (Pavo cristatus); Cryptosporidium avian genotype II in ostriches (Struthio camelus) and Cryptosporidium avian genotype III in a cockatiel (N. hollandicurs) and a peach-faced lovebird (Agapornis roseicolis). (C) 2009 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 05/59173-5 - Ocorrência e caracterização molecular de Cryptosporidium sp. em aves domésticas
• Beneficiário: Romulo Godik Antunes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 05/57625-6 - Ocorrência e caracterização molecular de cryptosporodium sp. em aves domésticas e aves exóticas mantidas em cativeiro
• Beneficiário: Alex Akira Nakamura
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/53809-5 - Ocorrência e caracterização molecular de Cryptospiridium sp. em aves domésticas e aves silvestres e exóticas mantidas em cativeiro
• Beneficiário: Marcelo Vasconcelos Meireles
• Modalidade de apoio: Auxílio à Pesquisa - Regular