Effect of Three Methods for Cleaning Dentures on Biofilms Formed In Vitro on Acrylic Resin

Autor(es)

Oliveira Paranhos, Helena F. ^[1] ; Silva-Lovato, Claudia H. ^[1] ; de Souza, Raphael F. ^[1] ; Cruz, Patricia C. ^[1] ; de Freitas-Pontes, Karina M. ^[1] ; Watanabe, Evandro ^[2] ; Ito, Izabel Y. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Dent Mat & Prosthodont, Ribeirao Preto Dent Sch, BR-14040904 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Clin Toxicol & Bromatol Anal, Fac Pharmaceut Sci Ribeirao Preto, BR-14040904 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY; v. 18, n. 5, p. 427-431, JUL 2009.
• Citações Web of Science: 35

Resumo

Purpose: The aim of this study was to evaluate the effect of three denture hygiene methods against different microbial biofilms formed on acrylic resin specimens. Materials and methods: The set (sterile stainless steel basket and specimens) was contaminated (37 degrees C for 48 hours) by a microbial inoculum with 106 colony-forming units (CFU)/ml (standard strains: Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, and Enterococcus faecalis; field strains: S. mutans, C. albicans, C. glabrata, and C. tropicalis). After inoculation, specimens were cleansed by the following methods: (1) chemical: immersion in an alkaline peroxide solution (Bonyplus tablets) for 5 minutes; (2) mechanical: brushing with a dentifrice for removable prostheses (Dentu Creme) for 20 seconds; and (3) a combination of chemical and mechanical methods. Specimens were applied onto a Petri plate with appropriate culture medium for 10 minutes. Afterward, the specimens were removed and the plates incubated at 37 degrees C for 48 hours. Results: Chemical, mechanical, and combination methods showed no significant difference in the reduction of CFU for S. aureus, S. mutans (ATCC and field strain), and P. aeruginosa. Mechanical and combination methods were similar and more effective than the chemical method for E. faecalis, C. albicans (ATCC and field strain), and C. glabrata. The combination method was better than the chemical method for E. coli and C. tropicalis, and the mechanical method showed intermediate results. Conclusion: The three denture hygiene methods showed different effects depending on the type of microbial biofilms formed on acrylic base resin specimens. (AU)

• Processo FAPESP: 02/12724-9 - Avaliação clínica e laboratorial de dois métodos de remoção do biofilme da dentadura
• Beneficiário: Helena de Freitas Oliveira Paranhos
• Modalidade de apoio: Auxílio à Pesquisa - Regular