Use of checkerboard DNA-DNA hybridization to evaluate the internal contamination of dental implants and comparison of bacterial leakage with cast or pre-machined abutments

Autor(es)

do Nascimento, Cassio ^[1] ; Santos Barbosa, Rodrigo Edson ^[1] ; Mardegan Issa, Joao Paulo ^[1] ; Watanabe, Evandro ^[2] ; Ito, Izabel Yoko ^[2] ; de Albuquerque, Jr., Rubens Ferreira ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Dent Ribeirao Preto, Dept Dent Mat & Prosthodont, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Toxicol & Bromatol Clin Anal, BR-14040904 Ribeirao Preto, SP - Brazil

^[3] McGill Univ, Fac Dent, Montreal, PQ - Canada

• Tipo de documento: Artigo Científico
• Fonte: Clinical Oral Implants Research; v. 20, n. 6, p. 571-577, JUN 2009.
• Citações Web of Science: 33

Resumo

To evaluate the checkerboard DNA-DNA hybridization method for detection and quantitation of bacteria from the internal parts of dental implants and to compare bacterial leakage from implants connected either to cast or to pre-machined abutments. Nine plastic abutments cast in a Ni-Cr alloy and nine pre-machined Co-Cr alloy abutments with plastic sleeves cast in Ni-Cr were connected to Branemark-compatible implants. A group of nine implants was used as control. The implants were inoculated with 3 mu l of a solution containing 10(8) cells/ml of Streptococcus sobrinus. Bacterial samples were immediately collected from the control implants while assemblies were completely immersed in 5 ml of sterile Tripty Soy Broth (TSB) medium. After 14 days of anaerobic incubation, occurrence of leakage at the implant-abutment interface was evaluated by assessing contamination of the TSB medium. Internal contamination of the implants was evaluated with the checkerboard DNA-DNA hybridization method. DNA-DNA hybridization was sensitive enough to detect and quantify the microorganism from the internal parts of the implants. No differences in leakage and in internal contamination were found between cast and pre-machined abutments. Bacterial scores in the control group were significantly higher than in the other groups (P < 0.05). Bacterial leakage through the implant-abutment interface does not significantly differ when cast or pre-machined abutments are used. The checkerboard DNA-DNA hybridization technique is suitable for the evaluation of the internal contamination of dental implants although further studies are necessary to validate the use of computational methods for the improvement of the test accuracy. To cite this article:do Nascimento C, Barbosa RES, Issa JPM, Watanabe E, Ito IY, Albuquerque Junior RF. Use of checkerboard DNA-DNA hybridization to evaluate the internal contamination of dental implants and comparison of bacterial leakage with cast or pre-machined abutments.Clin. Oral Impl. Res. 20, 2009; 571-577.doi: 10.1111/j.1600-0501.2008.01663.x. (AU)

• Processo FAPESP: 03/04585-1 - Avaliacao da contaminacao peri e intra-implantar e de proteses rosqueadas sobre implantes dentarios.
• Beneficiário: Rubens Ferreira de Albuquerque Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular