Electrocatalysis of reduced L-glutathione oxidation by iron(III) tetra-(N-methyl-4-pyridyl)-porphyrin (FeT4MPyP) adsorbed on multi-walled carbon nanotubes

Autor(es)

Luz, Rita C. S. ^[1] ; Damos, Flavio S. ^[2] ; Tanaka, Auro A. ^[3] ; Kubota, Lauro T. ^[1] ; Gushikem, Yoshitaka ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, BR-13084971 Campinas, SP - Brazil

^[2] Univ Fed Vales Jequitinhonha & Mucuri, Dept Chem, BR-39100000 Diamantina, MG - Brazil

^[3] Univ Fed Maranhao, Dept Chem, BR-65085580 Sao Luis, MA - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 76, n. 5, p. 1097-1104, SEP 15 2008.
• Citações Web of Science: 28

Resumo

The development of a highly sensitive voltammetric sensor for reduced L-glutathione (GSH) using a basal plane pyrolytic graphite (BPPG) electrode modified with iron(III) tetra-(N-methyl-4-pyridyl)-porphyrin (FeT4MPyP) adsorbed on multi-walled carbon nanotubes (MWCNT) is described. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were used to verify the morphologies and composition of the MWCNT after modification with the FeT4MPYP complex. The modified electrode showed very efficient electrocatalytic activity for L-glutathione oxidation, substantially decreasing the oxidation peak to -0.025 V vs Ag/AgCl. A linear response range from 5 mu mol L-1 to 5 mmol L-1 was obtained with a sensitivity of 703.41 mu A L mmol(-1). The detection limit for GSH determination was 0.5 mu mol L-1 and the relative standard deviation (R.S.D.) for 10 determinations of 250 mu mol L-1 GSH was 1.4%. The modified electrode was applied for GSH determination in erythrocyte samples and the results were in agreement to those obtained by a comparative method described in the literature. (C) 2008 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 00/11103-5 - Materiais porosos: preparação, propriedades e aplicações
• Beneficiário: Yoshitaka Gushikem
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/05185-5 - Desenvolvimento de sensores eletroquimicos a base de nanotubos de carbono midificados com ftalocianinas/porfirinas para determinacao de tiois: aplicacao em amostras biologicas, farmacologicas e alimentos.
• Beneficiário: Rita de Cássia Silva Luz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado