| Texto completo | |
| Autor(es): |
Pacheco, Richard
[1]
;
Rosa, Simone
[1]
;
Richtzenhain, Leonardo
[1]
;
Szabo, Matias P. J.
[2]
;
Labruna, Marcelo B.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Vet Med, Sao Paulo - Brazil
[2] Univ Fed Uberlandia, Fac Vet Med, BR-38400 Uberlandia, MG - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | REVISTA MVZ CORDOBA; v. 13, n. 2, p. 1273-1279, MAY-AUG 2008. |
| Citações Web of Science: | 16 |
| Resumo | |
Objective. To isolate and characterize rickettsiae from the ticks Amblyomma ovale and Amblyomma incisum collected in the state of Sao Paulo. Materials and methods. Adult, free-living A. ovale and A. incisum were collected in an Atlantic rainforest area in the state of Sao Paulo, Brazil. Each tick was tested using the hemolymph assay; samples from positive ticks were placed in shell vials in order to isolate rickettsiae and subsequently grown in Vero cells. Amplification of three rickettsial genes ( gltA, htrA and ompA) was attempted using polymerase chain reaction (PCR) for each isolate obtained. Amplicons were subsequently sequenced. Results. A total of 388 A. incisum and 50 A. ovale were collected. Only one A. incisum and one A. ovale were hemolymph-test positive. Rickettsiae were successfully isolated from these ticks; however establishment in Vero cell culture was successful only for the isolate from A. ovale. Bacterial contamination in the first cell passage of the A. incisum isolate precluded successful isolation of the organism. PCR products were obtained with the gltA and htrA primers for the two isolates, but no product was obtained with the ompA primers. By BLAST analysis, partial gltA and htrA sequences of isolates from A. ovale and A. incisum were similar to the corresponding sequences of R. bellii. Conclusions. This is the first report of R. bellii infecting A. incisum and the first successful isolation from A. ovale. (AU) | |
| Processo FAPESP: | 03/13872-4 - Rickettsia spp transmitidas por carrapatos nos Estados de São Paulo e Rondônia |
| Beneficiário: | Marcelo Bahia Labruna |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 03/13871-8 - Isolamento em cultivo de células e análises filogenética e taxonômica de uma riquétsia detectada em carrapatos Amblyomma cooperi, provenientes de áreas endêmicas para febre maculosa brasileira no estado de São Paulo |
| Beneficiário: | RICHARD DE CAMPOS PACHECO |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |