Gene expression modulation by heat stress in Acidithiobacillus ferrooxidans LR

Autor(es)

Ribeiro, Daniela A. ^[1] ; Ferraz, Lucio F. C. ^[1] ; Vicentini, Renato ^[1] ; Ottoboni, Laura M. M. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Ctr Mol Biol & Genet Engn CBMEG, BR-13083875 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY; v. 101, n. 3, p. 583-593, MAR 2012.
• Citações Web of Science: 0

Resumo

During bioleaching, Acidithiobacillus ferrooxidans is subjected to different types of stress, including heat stress, which affect bacterial growth. In this work, real time quantitative PCR was used to analyze the expression of heat shock genes, as well as genes that encode proteins related to several functional categories in A. ferrooxidans. Cells were submitted to long-term growth and heat shock, both at 40A degrees C. The results showed that heat shock affected the expression levels of most genes investigated, whilst long-term growth at 40A degrees C resulted in minor changes in gene expression, except for certain genes related to iron transport, which were strongly down-regulated, suggesting that the iron processing capability of A. ferrooxidans was affected by long-term growth at 40A degrees C. A bioinformatic analysis of the genes' promoter regions indicated a putative transcriptional regulation by the sigma(32) factor in 12 of the 31 genes investigated, suggesting the involvement of other regulatory mechanisms in the response of A. ferrooxidans to heat stress. (AU)

• Processo FAPESP: 02/07642-3 - Expressao diferencial de genes em acidithiobacillus ferrooxidans submetida ao estresse induzido por ph, temperatura e privacao de fosfato.
• Beneficiário: Laura Maria Mariscal Ottoboni
• Modalidade de apoio: Auxílio à Pesquisa - Regular