Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process

Autor(es)

Mattos, Ives B. ^[1] ; Alves, Danilo A. ^[1] ; Hollanda, Luciana M. ^[1] ; Ceragiogli, Helder J. ^[2] ; Baranauskas, Vitor ^[2] ; Lancellotti, Marcelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, LABIOTEC Biotechnol Lab, Dept Biochem, Inst Biol CP6109, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, NanoEng NanoEngn & Diamond Lab, Sch Elect & Comp Engn, Dept Semicond Instruments & Photon, UNICAMP, BR-13083852 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NANOBIOTECHNOLOGY; v. 9, NOV 16 2011.
• Citações Web of Science: 8

Resumo

Background: This study aimed at verifying the action of multi-walled carbon nanotubes (MWCNT) under the naturally transformable Neisseria meningitidis against two different DNA obtained from isogenic mutants of this microorganism, an important pathogen implicated in the genetic horizontal transfer of DNA, causing the escape of the principal vaccination measured worldwide by the capsular switching process. Materials and methods: The bacterium receptor strain C2135 was cultivated and had its mutant DNA donor M2 and M6, which received a receptor strain and MWCNT at three different concentrations. The inhibition effect of DNAse on the DNA in contact with nanoparticles was evaluated. Results: The results indicated an in increase in the transformation capacity of N. meninigtidis in different concentrations of MWCNT when compared with negative control without nanotubes. A final analysis of the interaction between DNA and MWCNT was carried out using Raman Spectroscopy. Conclusion: These increases in the transformation capacity mediated by MWCNT, in meningococci, indicate the interaction of these particles with the virulence acquisition of these bacteria, as well as with the increase in the vaccination escape process. (AU)

• Processo FAPESP: 08/56777-5 - Estudo molecular e profilático das bactérias naturalmente transformaveis do trato respiratório humano - Haemophilus influenzae, Neisseria meningitidis e Streptococcus pneumoniae
• Beneficiário: Marcelo Lancellotti
• Modalidade de apoio: Auxílio à Pesquisa - Regular