Taxonomic and Functional Microbial Signatures of the Endemic Marine Sponge Arenosclera brasiliensis

Autor(es):Mostrar menos -

Trindade-Silva, Amaro E. ^{[1, 2]} ; Rua, Cintia ^[2] ; Silva, Genivaldo G. Z. ^[3] ; Dutilh, Bas E. ^{[3, 4]} ; Moreira, Ana Paula B. ^[2] ; Edwards, Robert A. ^{[3, 5]} ; Hajdu, Eduardo ^[6] ; Lobo-Hajdu, Gisele ^[7] ; Vasconcelos, Ana Tereza ^[8] ; Berlinck, Roberto G. S. ^[1] ; Thompson, Fabiano L. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, Sao Carlos, SP - Brazil

^[2] Univ Fed Rio de Janeiro, Inst Biol, Rio De Janeiro - Brazil

^[3] San Diego State Univ, Dept Comp Sci, San Diego, CA 92182 - USA

^[4] Radboud Univ Nijmegen, Med Ctr, Nijmegen Ctr Mol Life Sci, Ctr Mol & Biomol Informat, NL-6525 ED Nijmegen - Netherlands

^[5] Argonne Natl Lab, Div Math & Comp Sci, Argonne, IL 60439 - USA

^[6] Univ Fed Rio de Janeiro, Museu Nacl, Dept Invertebrados, Rio De Janeiro - Brazil

^[7] Univ Estado Rio de Janeiro, Inst Biol Roberto Alcantara Gomes, BR-20550011 Rio De Janeiro - Brazil

^[8] Lab Nacl Comp Cient, Petropolis - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 7, n. 7 JUL 2 2012.
• Citações Web of Science: 35

Resumo

The endemic marine sponge Arenosclera brasiliensis (Porifera, Demospongiae, Haplosclerida) is a known source of secondary metabolites such as arenosclerins A-C. In the present study, we established the composition of the A. brasiliensis microbiome and the metabolic pathways associated with this community. We used 454 shotgun pyrosequencing to generate approximately 640,000 high-quality sponge-derived sequences (similar to 150 Mb). Clustering analysis including sponge, seawater and twenty-three other metagenomes derived from marine animal microbiomes shows that A. brasiliensis contains a specific microbiome. Fourteen bacterial phyla (including Proteobacteria, Cyanobacteria, Actinobacteria, Bacteroidetes, Firmicutes and Cloroflexi) were consistently found in the A. brasiliensis metagenomes. The A. brasiliensis microbiome is enriched for Betaproteobacteria (e.g., Burkholderia) and Gammaproteobacteria (e.g., Pseudomonas and Alteromonas) compared with the surrounding planktonic microbial communities. Functional analysis based on Rapid Annotation using Subsystem Technology (RAST) indicated that the A. brasiliensis microbiome is enriched for sequences associated with membrane transport and one-carbon metabolism. In addition, there was an overrepresentation of sequences associated with aerobic and anaerobic metabolism as well as the synthesis and degradation of secondary metabolites. This study represents the first analysis of sponge-associated microbial communities via shotgun pyrosequencing, a strategy commonly applied in similar analyses in other marine invertebrate hosts, such as corals and algae. We demonstrate that A. brasiliensis has a unique microbiome that is distinct from that of the surrounding planktonic microbes and from other marine organisms, indicating a species-specific microbiome. (AU)

• Processo FAPESP: 09/11612-1 - Caracterização do grupamento gênico de biossíntese de Arenosclerinas, alcaloides alquilpiperidínicos isolados da esponja Arenosclera brasiliensis
• Beneficiário: Amaro Emiliano Trindade Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado