| Texto completo | |
| Autor(es): Mostrar menos - |
Palhares, Alessandra C.
[1]
;
Rodrigues-Morais, Taislene B.
[1]
;
Van Sluys, Marie-Anne
[2]
;
Domingues, Douglas S.
[2]
;
Maccheroni, Jr., Walter
[3]
;
Jordao, Jr., Hamilton
[3]
;
Souza, Anete P.
[4]
;
Marconi, Thiago G.
[4]
;
Mollinari, Marcelo
[1]
;
Gazaffi, Rodrigo
[1]
;
Garcia, Antonio Augusto F.
[1]
;
Carneiro Vieira, Maria Lucia
[1]
Número total de Autores: 12
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba - Brazil
[2] Univ Sao Paulo, Dept Bot, Inst Biociencias, BR-05508090 Sao Paulo - Brazil
[3] CanaVialis Monsanto Co, BR-13069380 Campinas, SP - Brazil
[4] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BMC GENETICS; v. 13, JUN 28 2012. |
| Citações Web of Science: | 19 |
| Resumo | |
Background: The development of sugarcane as a sustainable crop has unlimited applications. The crop is one of the most economically viable for renewable energy production, and CO2 balance. Linkage maps are valuable tools for understanding genetic and genomic organization, particularly in sugarcane due to its complex polyploid genome of multispecific origins. The overall objective of our study was to construct a novel sugarcane linkage map, compiling AFLP and EST-SSR markers, and to generate data on the distribution of markers anchored to sequences of scIvana\_1, a complete sugarcane transposable element, and member of the Copia superfamily. Results: The mapping population parents ('IAC66-6' and `TUC71-7') contributed equally to polymorphisms, independent of marker type, and generated markers that were distributed into nearly the same number of co-segregation groups (or CGs). Bi-parentally inherited alleles provided the integration of 19 CGs. The marker number per CG ranged from two to 39. The total map length was 4,843.19 cM, with a marker density of 8.87 cM. Markers were assembled into 92 CGs that ranged in length from 1.14 to 404.72 cM, with an estimated average length of 52.64 cM. The greatest distance between two adjacent markers was 48.25 cM. The scIvana\_1-based markers (56) were positioned on 21 CGs, but were not regularly distributed. Interestingly, the distance between adjacent scIvana\_1-based markers was less than 5 cM, and was observed on five CGs, suggesting a clustered organization. Conclusions: Results indicated the use of a NBS-profiling technique was efficient to develop retrotransposon-based markers in sugarcane. The simultaneous maximum-likelihood estimates of linkage and linkage phase based strategies confirmed the suitability of its approach to estimate linkage, and construct the linkage map. Interestingly, using our genetic data it was possible to calculate the number of retrotransposonscIvana\_1 (similar to 60) copies in the sugarcane genome, confirming previously reported molecular results. In addition, this research possibly will have indirect implications in crop economics e. g., productivity enhancement via QTL studies, as the mapping population parents differ in response to an important fungal disease. (AU) | |
| Processo FAPESP: | 10/51708-5 - Mapas de ligação de cana de açúcar com base em marcadores derivados de retrotransposons, ETS-SSR, TRAP e AFLP e identificação de QTLs associados à resistência ao carvão |
| Beneficiário: | Maria Lúcia Carneiro Vieira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |