Analysis of the protective potential of antigens released by Leishmania (Viannia) shawi promastigotes

Autor(es)

Passero, Luiz Felipe Domingues ^[1] ; Marques, Claudia ^[2] ; Vale-Gato, Ines ^[2] ; Corbett, Carlos Eduardo Pereira ^[1] ; Laurenti, Marcia Dalastra ^[1] ; Santos-Gomes, Gabriela ^{[3, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Lab Patol Molestias Infecciosas, Sao Paulo - Brazil

^[2] Univ Nova Lisboa, Inst Higiene & Med Trop, Ctr Malaria & Outras Doencas Trop, Unidade Ensino & Invest Parasitol Med, P-1200 Lisbon - Portugal

^[3] Ctr Malaria & Outras Doencas Trop, IHMT, Unidade Ensinno & Invest Parasitol Med, P-1349008 Lisbon - Portugal

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF DERMATOLOGICAL RESEARCH; v. 304, n. 1, p. 47-55, JAN 2012.
• Citações Web of Science: 3

Resumo

Leishmania (Viannia) shawi causes cutaneous lesions in humans. Parasite antigens conferring significant protection against American tegumentar leishmaniosis (ATL) might be important for the development of effective vaccine. Therefore, this work evaluates the protective effect of antigenic fractions released by L. shawi. Antigens released by promastigotes to culture medium were concentrated and isolated by SDS-PAGE. The three main fractions LsPass1 (>75 kDa), LsPass2 (75-50 kDa) and LsPass3 (<50 kDa) were electro-eluted according with their molecular mass. Immunized BALB/c mice were challenged with L. shawi promastigotes and the course of infection monitored during 5 weeks. LsPass1-challenged mice showed no protection, however, a strong degree of protection associated to smaller lesions and high expression of IFN-gamma and TNF-alpha by CD4(+) T, CD8(+) T and double negative CD4CD8 cells was achieved in LsPass3-challenged mice. Furthermore, LsPass2-challenged mice showed an intermediated degree of protection associated to high levels of IFN-gamma, IL-4 and IL-10 mRNA. In spite of increased expression of IFN-gamma and TNF-alpha, high amounts of IL-4 and IL-10 mRNA were also detected in LsPass3-challenged mice indicating a possible contribution of these cytokines for the persistence of a residual number of parasites that may be important in inducing long-lasting immunity. Therefore, LsPass3 seems to be an interesting alternative that should be considered in the development of an effective vaccine against ATL. (AU)

• Processo FAPESP: 07/56209-4 - Avaliação da pesquisa imune celular desencadeada por antígenos proteicos isolados de Leishmania (Viannia) shawi
• Beneficiário: Marcia Dalastra Laurenti
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/50654-6 - Avaliação da resposta imune celular desencadeada por antígenos protéicos isolados de Leishmania (Viannia) shawi
• Beneficiário: Luiz Felipe Domingues Passero
• Linha de fomento: Bolsas no Brasil - Doutorado Direto