Bacterial cellulose-collagen nanocomposite for bone tissue engineering

Autor(es)

Saska, Sybele ^[1] ; Teixeira, Lucas Novaes ^[2] ; de Oliveira, Paulo Tambasco ^[2] ; Minarelli Gaspar, Ana Maria ^[3] ; Lima Ribeiro, Sidney Jose ^[1] ; Messaddeq, Younes ^[1] ; Marchetto, Reinaldo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Unesp, Inst Chem, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Dent Ribeirao Preto, Cell Culture Lab, BR-14040904 Sao Paulo - Brazil

^[3] Univ Estadual Paulista UNESP, Sch Dent, Dept Morphol, BR-14801903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Materials Chemistry; v. 22, n. 41, p. 22102-22112, NOV 7 2012.
• Citações Web of Science: 60

Resumo

A nanocomposite based on bacterial cellulose (BC) and type I collagen (COL) was evaluated for in vitro bone regeneration. BC membranes were modified by glycine esterification followed by cross-linking of type I collagen employing 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. Collagen incorporation was studied by spectroscopy analysis. X-Ray diffraction showed changes in the BC crystallinity after collagen incorporation. The elastic modulus and tensile strength for BC-COL decreased, while the strain at failure showed a slight increase, even after sterilization, as compared to pristine BC. Swelling tests and contact angle measurements were also performed. Cell culture experiments performed with osteogenic cells were obtained by enzymatic digestion of newborn rat calvarium revealed similar features of cell morphology for cultures grown on both membranes. Cell viability/proliferation was not different between BC and BC-COL membranes at day 10 and 14. The high total protein content and ALP activity at day 17 in cells cultured on BC-COL indicate that this composite allowed the development of the osteoblastic phenotype in vitro. Thus, BC-COL should be considered as alternative biomaterial for bone tissue engineering. (AU)

• Processo FAPESP: 09/09960-1 - Nanocompositos à base de celulose bacteriana para aplicação na regeneração do tecido ósseo.
• Beneficiário: Reinaldo Marchetto
• Modalidade de apoio: Auxílio à Pesquisa - Regular