Cytotoxic effects of andiroba oil (Carapa guianensis) in reproductive system of Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) semi-engorged females

Autor(es)

Ramalho Vendramini, Maria Claudia ^[1] ; Camargo-Mathias, Maria Izabel ^[1] ; de Faria, Adriano Uemura ^[1] ; Bechara, Gervasio Henrique ^[1] ; de Oliveira, Patricia Rosa ^[1] ; Roma, Gislaine Cristina ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology Research; v. 111, n. 5, p. 1885-1894, NOV 2012.
• Citações Web of Science: 21

Resumo

The present study performed an analysis about the effects of andiroba seed oil (Carapa guianensis) in the ovary of Rhipicephalus sanguineus semi-engorged females; once, there are few studies about the action of natural products on the reproductive system, a vital organ for the biological success of this animal group. The results showed that andiroba oil is a potent natural agent which causes significant structural changes in the oocytes, such as the emergence of large vacuolated cytoplasmic regions, reduction in the number of yolk granules, changes in the shape of the cells, as well as impairment of genetic material. In addition, the ovary epithelium showed severe morphological changes, such as extreme structural disorganization, with highly vacuolated cells and picnotic nuclei, forming an amorphous mass. This study showed also that oocytes (mainly in the initial stages of development) and the ovary epithelium of R. sanguineus females subjected to different concentrations of andiroba oil presented morphological changes which became more numerous and intense as the concentration of the product increased. Based on the results, it can be inferred that although the defense mechanisms are developed by oocytes to recover the cellular integrity (presence of autophagic vacuoles), these cells are not able to revert the damage caused by this product. Thus, it can be concluded that although the damages caused to the oocytes by andiroba oil are comparatively less severe than the ones caused by synthetic acaricides, this product can be considered a potent natural agent that reduce and/or prevent the reproduction of R. sanguineus females, with the advantage of not causing environmental impact such as synthetic chemical acaricides. (AU)

• Processo FAPESP: 11/10427-6 - Auxílio técnico para manutenção de colônias de carrapatos e processamento de técnicas laboratoriais
• Beneficiário: Mateus Ribeiro Mota
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 11/07938-9 - Avaliação das alterações celulares nos ovócitos de fêmeas semi-ingurgitadas de Carrapatos Rhipicephalus sanguineus (Acari: Ixodidae) decorrentes da exposição ao óleo de andiroba (Carapa guianensis)
• Beneficiário: Maria Claudia Ramalho Vendramini
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/06865-8 - Estudo morfofisiológico comparativo do sistema nervoso de larvas, ninfas e adultos de carrapatos Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) e avaliação celular do singânglio de fêmeas semi-ingurgitadas expostas à permetrina e ao óleo de andiroba
• Beneficiário: Gislaine Cristina Roma
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 10/51942-8 - Estudo morfofisiológico comparativo do sistema nervoso de larvas, ninfas e adultos de carrapatos Rhipicephalus sanguineus (Acari: Ixodidae) e avaliação celular do singânglio de fêmeas semi-ingurgitadas expostas à permetrina e ao óleo de andiroba (Carapa guianensis)
• Beneficiário: Gislaine Cristina Roma
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores