Haplotypes of susceptibility to chronic periodontitis in the Interleukin 8 gene do not influence protein level in the gingival crevicular fluid

Autor(es):Mostrar menos -

Corbi, Samia C. T. ^[1] ; Anovazzi, Giovana ^[1] ; Finoti, Livia S. ^[1] ; Kim, Yeon J. ^[1] ; Capela, Marisa V. ^[2] ; Secolin, Rodrigo ^[3] ; Marcaccini, Andrea M. ^[4] ; Gerlach, Raquel F. ^[4] ; Orrico, Silvana R. P. ^[1] ; Cirelli, Joni A. ^[1] ; Scarel-Caminaga, Raquel M. ^[5]

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, Sch Dent Araraquara, Dept Oral Diag & Surg, Araraquara, SP - Brazil

^[2] UNESP Sao Paulo State Univ, Inst Chem Araraquara, Dept Phys Chem, Araraquara, SP - Brazil

^[3] Univ Campinas UNICAMP, Dept Med Genet, Campinas, SP - Brazil

^[4] Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Stomatol & Physiol, Ribeirao Preto, SP - Brazil

^[5] UNESP Sao Paulo State Univ, Sch Dent Araraquara, Dept Morphol, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 57, n. 10, p. 1355-1361, OCT 2012.
• Citações Web of Science: 10

Resumo

Objective: Previously, we identified that the ATC/TTC haplotype formed by polymorphisms in the Interleukin-(IL)8 gene conferred susceptibility to chronic periodontitis (CP). The aim of the study was to investigate whether the IL8 haplotype ATC/TTC was associated with the volume of gingival crevicular fluid (GCF), the concentration of interleukin IL-8 in the GCF, as well as periodontal conditions in patients with CP in comparison to controls without CP. Methods: Seventy-nine individuals (CP: n = 41, controls: n = 38) were grouped according to the presence (susceptible for CP) or absence (not susceptible for CP) of the IL8 ATC/TTC haplotype. After periodontal clinical evaluation, they were subdivided by the presence or absence of CP. GCF was collected from each patient and the IL-8 levels were determined by ELISA. The GCF volume of each subject was measured by means of a calibrated electronic device. Comparisons of means between carriers and non-carriers of the ATC/TTC haplotype were evaluated using the Mann-Whitney test. Linear regression and stepwise linear regression analysis were used to analyse the association of the GCF volume with potential covariates and their contribution for the phenotype. Results: We did not find significant differences of both periodontal conditions and IL-8 concentration in the GCF of patients with the presence or absence of the IL8 ATC/TTC haplotype. However, the GCF volume was significantly higher amongst the patients affected by CP that are absent for the IL8 ATC/TTC haplotype. In addition, linear regression analysis showed a statistically significant association between GCF volume and CP, IL8 haplotype ATC/TTC and IL-8 concentration. Conclusions: The IL8 haplotype of susceptibility to CP was neither associated with IL-8 cytokine levels nor with clinical periodontal parameters. Also, CP, IL8 haplotype and IL-8 concentration showed a positive association with the GCF volume levels in the studied patients. (c) 2012 Published by Elsevier Ltd. (AU)

• Processo FAPESP: 09/08773-3 - Análises clínica, microbiológica e imunológica em indivíduos com suscetibilidade genética à doença periodontal
• Beneficiário: Raquel Mantuaneli Scarel Caminaga
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/10424-0 - Investigação de polimorfismos no gene interleucina 8 e seus receptores em indivíduos com doença periodontal crônica
• Beneficiário: Raquel Mantuaneli Scarel Caminaga
• Modalidade de apoio: Auxílio à Pesquisa - Regular