Toxicity of trifluralin herbicide in a representative of the edaphic fauna: Histopathology of the midgut of Rhinocricus padbergi (Diplopoda)

Autor(es)

Merlini, Vanessa Vanderleia ^[1] ; Nogarol, Larissa Rosa ^[1] ; Marin-Morales, Maria Aparecida ^[1] ; Fontanetti, Carmem Silvia ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Dept Biol, BR-13506900 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 75, n. 10, p. 1361-1369, OCT 2012.
• Citações Web of Science: 6

Resumo

The aim of this study was to use the diplopod Rhinocricus padbergi as test organism to verify the possible toxic effects of the herbicide trifluralin in a representative of the edaphic fauna. Histological and histochemical techniques were applied in the midgut of the animals after exposure to different concentrations of trifluralin for 7 and 90 days (acute and subchronic exposure, respectively). The individuals from the control group did not present alterations to any of the exposures. In the acute exposure, the group exposed to a quarter and half of the field dose presented an accumulation of cytoplasmic granules in the hepatic cells; the field dose group presented an increase in the rate of epithelial renewal and the group exposed to double the field concentration presented an increase in the release of secretory vesicles. The subchronic response was obtained with the field dose and double field dose because the animals from a quarter and half groups did not survive until the end of the experiment. In both groups, the animals presented disruption of the epithelium and higher occurrence of hemocytes among the hepatic cells. The group exposed to double the field dose presented sites of cytoplasmic vacuolization, dilatation of the intercellular space, increase in the epithelial renewal, and release of secretory vesicles. The results alert for a careful use of this herbicide, since it promoted alterations in the midgut cells of the test organism used. Microsc. Res. Tech. 2012. (c) 2012 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 10/09588-2 - Avaliação do efeito tóxico do herbicida trifluralina por meio do estudo histológico e histoquímico do intestino médio do diplopodo Rhinocricus padbergi
• Beneficiário: Vanessa Vanderléia Merlini
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica