Platelet-rich plasma stimulates cytokine expression and alkaline phosphatase activity in osteoblast-derived osteosarcoma cells

Autor(es)

Herrera, Bruno S. ^[1] ; Coimbra, Leila S. ^[1] ; Bastos, Alliny S. ^[2] ; Teixeira, Simone A. ^[3] ; Steffens, Joao P. ^[1] ; Muscara, Marcelo N. ^[3] ; Spolidorio, Luis C. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Dept Physiol & Pathol, FOAr, BR-14801903 Araraquara, SP - Brazil

^[2] UNESP, Dept Diag & Surg, FOAr, BR-14801903 Araraquara, SP - Brazil

^[3] Univ Sao Paulo, ICB, Dept Pharmacol, BR-09500900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 57, n. 9, p. 1282-1289, SEP 2012.
• Citações Web of Science: 4

Resumo

Objective: The aim of this study was to investigate the effects of PRP on SAOS-2 cells in terms of cytokine expression, cell activity and oxidative stress. Design: Cell line SAOS-2 (1 x 10(5) cells/mL) were grown in culture medium alpha-MEM with 10% FBS for 24 h and stimulated (or not) with PRP at concentrations of 3, 10 and 20%, LPS (E. coli, 10 g/mL) and IL-1 beta (1 mg/mL) for 24 h. The supernatant was collected and analyzed for the expression of cytokines in a panel array, ALP using a commercial kit and NO2- with Griess reaction method. Also, the cells were analyzed using Western blot for RANKL and slot blotting for nitrotyrosine expression. Result: There were no significant differences amongst the groups in terms of NO2-, protein nitrotyrosine content and RANKL expression. However, all stimuli increased ALP activity and in case of PRP, it was in a dose-dependent manner (p < 0.001). Also, all stimuli induced an increase in cytokines and chemokines expression, but only PRP promoted an increase of component C5, sICAM-1 and RANTES expression. Whilst IL-1 receptor antagonist (IL-1ra) expression was down-regulated by PRP, both LPS and IL-1 beta caused up-regulation of this cytokine. Conclusions: PRP can stimulate osteoblast activity and cytokine/chemokine release, as well as indicate some of the mediators that can (and cannot) be involved in this activation. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/02893-4 - Estabelecimento e padronização de culturas de osteoclastos e osteoblastos humanos como ferramenta para o estudo dos mecanismos envolvidos na perda óssea alveolar decorrente da periodontite
• Beneficiário: Bruno Schneider Herrera
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado