Analysis by confocal laser scanning microscopy of the MDPB bactericidal effect on S. mutans biofilm CLSM analysis of MDPB bactericidal effect on biofilm

Autor(es)

de Carvalho, Fabiola Galbiatti ^[1] ; Puppin-Rontani, Regina Maria ^[2] ; Portugal de Fucio, Suzana Beatriz ^[3] ; Negrini, Thais de Cassia ^[4] ; Carlo, Hugo Lemes ^[5] ; Garcia-Godoy, Franklin ^[6]

Afiliação do(s) autor(es)

^[1] Univ Fed Campina Grande, Hlth & Technol Rural Ctr, Curso Odontol, BR-58708110 Patos de Minas, PB - Brazil

^[2] Univ Estadual Campinas, Sch Dent Piracicaba, Dept Pediat Dent, Piracicaba, SP - Brazil

^[3] Univ Estadual Campinas, Sch Dent Piracicaba, Dent Mat Area, Dept Restorat Dent, Piracicaba, SP - Brazil

^[4] Univ Estadual Campinas, Dept Microbiol, Sch Dent Piracicaba, Piracicaba, SP - Brazil

^[5] Univ Fed Paraiba, Hlth Sci Ctr, Dept Operat Dent, BR-58059900 Joao Pessoa, Paraiba - Brazil

^[6] Univ Tennessee, Coll Dent, Biosci Res Ctr, Memphis, TN - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 20, n. 5, p. 568-575, SEP-OCT 2012.
• Citações Web of Science: 25

Resumo

Since bacteria remain in the dentin following caries removal, restorative materials with antibacterial properties are desirable to help maintaining the residual microorganisms inactive. The adhesive system Clearfil Protect Bond (PB) contains the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) in its primer, which has shown antimicrobial activity. However, its bactericidal effect against biofilm on the dentin has been little investigated. Objective: The aim of this study was to analyze by confocal laser scanning microscopy (CLSM) and viable bacteria counting (CFU) the MDPB bactericidal effect against S. mutans biofilm on the dentin surface. Material and Methods: Bovine dentin surfaces were obtained and subjected to S. mutans biofilm formation in BHI broth supplemented with 1% (w/v) sucrose for 18 h. Samples were divided into three groups, according to the primer application (n=3): Clearfil Protect Bond (PB), Clearfil SE Bond, which does not contain MDPB, (SE) and saline (control group). After the biofilm formation, Live/Dead stain was applied directly to the surface of each sample. Next, 10 mu L of each primer were applied on the samples during 590 s for the real-time CLSM analysis. The experiment was conducted in triplicate. The primers and saline were also applied on the other dentin samples during 20, 90, 300 and 590 s (n=9 for each group and period evaluated) and the CFU were assessed by colonies counting. Results: The results of the CLSM showed that with the SE application, although non-viable bacteria were detected at 20 s, there was no increase in their count during 590 s. In contrast, after the PB application there was a gradual increase of non-viable bacteria over 590 s. Conclusions: The quantitative analysis demonstrated a significant decrease of S. mutans CFU at 90 s PB exposure and only after 300 s of SE application. Protect Bond showed an earlier antibacterial effect than SE Bond. (AU)

• Processo FAPESP: 05/57268-9 - Desempenho de sistemas de união à dentina decídua e permanente afetada por cárie quando submetidos ao desafio cariogênico: análise mecânica e micromorfológica
• Beneficiário: Fabíola Galbiatti de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Doutorado