Immune Cells Depletion During Wound Healing as a Long-Term Effect of Undernutrition

Autor(es)

Marcelo Zuanassi Macari ^[1] ; Fabiano Misael Cavenaghi ^[2] ; Marilena Chinali Komesu ^[3] ; Laurelúcia Orive Lunardi ^[4] ; Miguel Angel Sala ^[5] ; Arthur Belém Novaes Júnior ^[6] ; Márcio Fernando de Moraes Grisi ^[7] ; Mário Taba Júnior ^[8] ; Sérgio Luiz Scombatti de Souza ^[9]

Afiliação do(s) autor(es)

^[1] University of São Paulo. School of Dentistry. Stomatology and Physiology, Ribeirão Preto - Brasil

^[2] University of São Paulo. School of Dentistry. Stomatology and Physiology, Ribeirão Preto - Brasil

^[3] University of São Paulo. School of Dentistry. Stomatology and Physiology, Ribeirão Preto - Brasil

^[4] University of São Paulo. School of Dentistry. Stomatology and Physiology, Ribeirão Preto - Brasil

^[5] University of São Paulo. School of Dentistry. Stomatology and Physiology, Ribeirão Preto - Brasil

^[6] University of São Paulo. School of Dentistry. Department of Oral Surgery - Brasil

^[7] University of São Paulo. School of Dentistry. Department of Oral Surgery - Brasil

^[8] University of São Paulo. School of Dentistry. Department of Oral Surgery - Brasil

^[9] University of São Paulo. School of Dentistry. Department of Oral Surgery - Brasil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MORPHOLOGY; v. 23, n. 1, p. 25-32, 2005-00-00.

Resumo

Undernutrition in early life is associated with a number of acute and chronic sequelae, and recovering is a controversial issue. Even if undernutrition in Brazil is declining, studies have shown that about 31% of brazilian children still present severe or moderate malnutrition. The present study goal was to induce early malnutrition in rats and observe short- (undernourished) and long-term (after recovered) effects on defense cells involved in wound healing. Undernutrition was produced by separating the pups from the mother for 10 hours/day during the suckling period (21 days after birth). As controls were used rats at same age not submitted to suckling restriction. Undernutrition and recovering states were assessed by body weight. Skin wounds were made on the shaved backs of all, undernourished, recovered and their controls, under tribromoethanol anesthesia. Aninals were sacrificed 1, 3, 7 and 14 days after surgery and the tissues were properly prepared and observed under light microscopy. Our results showed that: 1) neutrophils, lymphocytes and macrophages couting in healing area were lower in undernourished and in recovered animals as compared with their controls; 2) there were a basal deficiency in lymphocytes and macrophages numbers in recovered animals but not in those acutelly undernourished. These results allow us to conclude that post-nattally undernourished animals submitted to a nutritional rescue time showed a complete recovery in physical weight, but in spite of the physical recovery, the wound healing showed less defense cell density in healing areas suggesting long-term sequelae of early undernutrition (AU)

• Processo FAPESP: 98/04721-2 - Efeito da desnutrição pós-natal sobre a capacidade de reparo e cicatrização da ferida cirúrgica no rato
• Beneficiário: Marilena Chinali Komesu
• Modalidade de apoio: Auxílio à Pesquisa - Regular