| Texto completo | |
| Autor(es): |
Valenti, Laura E.
[1]
;
Martins, Vitor L.
[2]
;
Herrera, Elisa
[1]
;
Torresi, Roberto M.
[2]
;
Giacomelli, Carla E.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Nacl Cordoba, Inst Invest Fis Quim Cordoba INFIQC CONICET UNC, Fac Ciencias Quim, Dept Quim Fis, RA-5000 Cordoba - Argentina
[2] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF MATERIALS CHEMISTRY B; v. 1, n. 38, p. 4921-4931, 2013. |
| Citações Web of Science: | 12 |
| Resumo | |
This work investigated a simple and versatile modification to a solid substrate to develop electrochemical bio-recognition platforms based on bio-affinity interactions between histidine (His)-tagged proteins and Ni(II) surface sites. Carboxylate (COO)-functionalized substrates were prepared in multiple steps, initiated with an amino-terminated self-assembled monolayer (SAM) on polycrystalline gold. Surface enhanced Raman spectroscopy (SERS), quartz crystal microbalance with dissipation monitoring (QCM-D) and contact angle measurements were used to follow the modification process. Upon completion of the modification process, the surface COO-Ni(II) chelate complex and the coordination mode used to bind the His-tag proteins were characterized by X-ray absorption near-edge spectroscopy (XANES). Finally, the electrochemical stability and response of the modified substrates were evaluated. The versatility of the modification process was verified using silica as the substrate. QCM-D and SERS results indicated that two types of films were formed: a COO-terminated SAM, which resulted from the reduction of previously incorporated surface aldehyde groups, and a physically adsorbed polymeric glutaraldehyde film, which was produced in the alkaline medium. XANES spectral features indicated that COO-Ni(II) formed a non-distorted octahedral complex on the substrate. The electrochemical stability and response towards a redox mediator of the COO-Ni(II)-terminated SAM indicated that this platform could be easily coupled to an electrochemical method to detect bio-recognition events. (AU) | |
| Processo FAPESP: | 09/53199-3 - Reconhecimento molecular e armazenamento de energia: estudos fundamentais do efeito de geometria, tamanho e síntese na otimização das propriedades químicas de materiais eletroativos |
| Beneficiário: | Roberto Manuel Torresi |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 09/09209-4 - Eletrodeposição de metais utilizando líquidos iônicos |
| Beneficiário: | Vitor Leite Martins |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |