The effect of nitrate assimilation deficiency on the carbon and nitrogen status of Arabidopsis thaliana plants

Autor(es)

Santos-Filho, Plinio Rodrigues ^[1] ; Saviani, Elzira Elisabeth ^[1] ; Salgado, Ione ^[1] ; Oliveira, Halley Caixeta ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Inst Biol, Dept Plant Biol, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Amino Acids; v. 46, n. 4, p. 1121-1129, APR 2014.
• Citações Web of Science: 2

Resumo

Carbon (C) and nitrogen (N) metabolism are integrated processes that modulate many aspects of plant growth, development, and defense. Although plants with deficient N metabolism have been largely used for the elucidation of the complex network that coordinates the C and N status in leaves, studies at the whole-plant level are still lacking. Here, the content of amino acids, organic acids, total soluble sugars, starch, and phenylpropanoids in the leaves, roots, and floral buds of a nitrate reductase (NR) double-deficient mutant of Arabidopsis thaliana (nia1 nia2) were compared to those of wild-type plants. Foliar C and N primary metabolism was affected by NR deficiency, as evidenced by decreased levels of most amino acids and organic acids and total soluble sugars and starch in the nia1 nia2 leaves. However, no difference was detected in the content of the analyzed metabolites in the nia1 nia2 roots and floral buds in comparison to wild type. Similarly, phenylpropanoid metabolism was affected in the nia1 nia2 leaves; however, the high content of flavonol glycosides in the floral buds was not altered in the NR-deficient plants. Altogether, these results suggest that, even under conditions of deficient nitrate assimilation, A. thaliana plants are capable of remobilizing their metabolites from source leaves and maintaining the C-N status in roots and developing flowers. (AU)

• Processo FAPESP: 08/11636-5 - Sinalização do óxido nítrico (NO) na resposta de defesa de plantas ao ataque de patógenos: análise da expressão gênica, produção de compostos secundários e bioenergética mitocondrial
• Beneficiário: Ione Salgado
• Modalidade de apoio: Auxílio à Pesquisa - Regular