The trypsin inhibitor from Entada acaciifolia seeds affects negatively the development of Mediterranean flour moth, Anagasta kuehniella

Autor(es)

Ramalho de Oliveira, Caio Fernando ^{[1, 2]} ; Marangoni, Sergio ^[1] ; Rodrigues Macedo, Maria Ligia ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem, BR-13083970 Campinas, SP - Brazil

^[2] Univ Mato Grosso Sul, Ctr Biol & Hlth Sci, Dept Food Technol & Publ Hlth, BR-79070900 Campo Grande, MS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY; v. 108, p. 74-79, JAN 2014.
• Citações Web of Science: 12

Resumo

The Mediterranean flour moth (Anagasta kuehniella) is a pest insect that attacks stored foods. The difficulty in controlling this kind of pest promotes the development of alternatives for pest control, among them the use of proteins with insecticide effect. In this work, we evaluated the role of a trypsin inhibitor purified from Entada acaciifolia seeds (EATI) on the A. kuehniella development. Different concentrations of inhibitor were added to a diet to determine its effects on insect performance. At 0.4%, the EATI decreases the larval weight and survival rates by 54.6% and 15%, respectively; in addition to the extension of the life cycle of insect. The biochemical analysis showed that the inhibitor is refractory to the digestion by midgut proteases, and led to a reduction of 32% in general proteolytic activity. A detailed analysis of the enzymatic activity revealed a decrease of 50% in trypsin activity as the chymotrypsin activity increased by 12%; possibly to compensate the commitment of the digestive process. The trypsins from the EATI-fed group stayed sensitive to the inhibition by EATI, and based on kinetic assays no new trypsin enzymes were produced as adaptation attempt. The insecticides effects observed for the EATI against this pest encourage a more in depth study of its possible long-term use as a biotechnological tool. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/09361-0 - Inibidor de tripsina de Entada acaciifolia (EATI): caracterização estrutural e avaliação do potencial inseticida contra a lagarta-do-cartucho (Spodoptera frugiperda)
• Beneficiário: Sergio Marangoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular