Evolutionarily conserved morphogenetic movements at the vertebrate head-trunk interface coordinate the transport and assembly of hypopharyngeal structures

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Lours-Calet, Corinne ^{[1, 2]} ; Alvares, Lucia E. ^{[1, 3]} ; El-Hanfy, Amira S. ^[1] ; Gandesha, Saniel ^{[1, 4]} ; Walters, Esther H. ^[1] ; Sobreira, Debora Rodrigues ^{[3, 5]} ; Wotton, Karl R. ^{[1, 6, 7]} ; Jorge, Erika C. ^{[1, 8]} ; Lawson, Jennifer A. ^[9] ; Lewis, A. Kelsey ^[9] ; Tada, Masazumi ^[10] ; Sharpe, Colin ^[11] ; Kardon, Gabrielle ^[9] ; Dietrich, Susanne ^{[1, 5]}

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^[1] Kings Coll London, Sch Biomed & Hlth Sci, London SE1 1UL - England

^[2] Clermont Univ, GReD Genet Reprod & Dev, UMR CNRS 6247, INSERM U931, F-63171 Aubiere - France

^[3] Univ Campinas UNICAMP, Dept Histol & Embryol, BR-13083863 Campinas, SP - Brazil

^[4] Coll Rd Dent Practice, Bromsgrove B60 2NE - England

^[5] Univ Portsmouth, Sch Pharm & Biomed Sci, IBBS, Portsmouth PO1 2DT, Hants - England

^[6] CRG, EMBL, Syst Biol Res Unit, Barcelona 08003 - Spain

^[7] UPF, Barcelona 08003 - Spain

^[8] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Morphol, Dept Morfol, Belo Horizonte, MG - Brazil

^[9] Univ Utah, Dept Human Genet, Salt Lake City, UT 84112 - USA

^[10] UCL, Dept Cell & Dev Biol, London WC1E 6BT - England

^[11] Univ Portsmouth, Sch Biol, IBBS, Portsmouth PO1 2DT, Hants - England

• Tipo de documento: Artigo Científico
• Fonte: Developmental Biology; v. 390, n. 2, p. 231-246, JUN 15 2014.
• Citações Web of Science: 19

Resumo

The vertebrate head trunk interface (occipital region) has been heavily remodelled during evolution, and its development is still poorly understood. In extant jawed vertebrates, this region provides muscle precursors for the throat and tongue (hypopharyngeal/hypobranchial/hypoglossal muscle precursors, HMP) that take a stereotype path rostrally along the pharynx and are thought to reach their target sites via active migration. Yet, this projection pattern emerged in jawless vertebrates before the evolution of migratory muscle precursors. This suggests that a so far elusive, more basic transport mechanism must have existed and may still be traceable today. Here we show for the first time that all occipital tissues participate in well-conserved cell movements. These cell movements are spearheaded by the occipital lateral mesoderm and ectoderm that split into two streams. The rostrally directed stream projects along the floor of the pharynx and reaches as far rostrally as the floor of the mandibular arch and outflow tract of the heart. Notably, this stream leads and engulfs the later emerging HMP, neural crest cells and hypoglossal nerve. When we (i) attempted to redirect hypobranchial/hypoglossal muscle precursors towards various attractants, (ii) placed non-migratory muscle precursors into the occipital environment or (iii) molecularly or (iv) genetically rendered muscle precursors non-migratory, they still followed the trajectory set by the occipital lateral mesoderm and ectoderm. Thus, we have discovered evolutionarily conserved morphogenetic movements, driven by the occipital lateral mesoderm and ectoderm, that ensure cell transport and organ assembly at the head trunk interface. (C) 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). (AU)

• Processo FAPESP: 06/05892-3 - Estudo das funções dos genes da família Frodo/Dapper na embriogênese dos vertebrados
• Beneficiário: Lúcia Elvira Álvares
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores