Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Coriandrum sativum L. (Coriander) Essential Oil: Antifungal Activity and Mode of Action on Candida spp., and Molecular Targets Affected in Human Whole-Genome Expression

Texto completo
Autor(es):
Freires, Irlan de Almeida [1] ; Murata, Ramiro Mendonca [2, 3] ; Furletti, Vivian Fernandes [4] ; Sartoratto, Adilson [4] ; de Alencar, Severino Matias [5] ; Figueira, Glyn Mara [4] ; de Oliveira Rodrigues, Janaina Aparecida [6] ; Teixeira Duarte, Marta Cristina [4] ; Rosalen, Pedro Luiz [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil
[2] Univ So Calif, Ostrow Sch Dent, Div Periodontol Diagnost Sci & Dent Hyg, Los Angeles, CA - USA
[3] Univ So Calif, Ostrow Sch Dent, Div Biomed Sci, Los Angeles, CA - USA
[4] Univ Estadual Campinas, Res Ctr Chem Biol & Agr, Campinas, SP - Brazil
[5] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agri Food Ind Food & Nutr, Piracicaba, SP - Brazil
[6] Univ Sao Paulo, Sch Engn Sao Carlos, Postgrad Program Interunits Bioengn, Sao Carlos, SP - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 9, n. 6 JUN 5 2014.
Citações Web of Science: 36
Resumo

candidiasis is an opportunistic fungal infection of the oral cavity with increasingly worldwide prevalence and incidence Novel specifically-targeted strategies to manage this ailment have been proposed using essential oils CEO) known to have antifungal properties. In this study, we aim to investigate the antifungal activity and mode of action of the EO from Coriandrum sativurn L (coriander) leaves on Candida spp. In addition detected the molecular targets affected in who genorne expression in human cells. The EO phytochernical profile indicates monoterpenes and sesquiterpenes as components, which are likely to negatively impact the viability of yeast cells. There seems to be a synergistic activity of EO chemical compounds as their isolation into fractions led to a decreased antimicrobial effect. C. sativum EO may bind membrane ergosterol, increasing ionic permeability and causing membrane damage leading to cell death, but it does not on cell wall biosynthesis-related pathways. This mode of action is illustrated by photomicrographs showing disruption in caused by the EO at varied concentrations. The EO also inhibited Candida biofilm adherence to polystyrene substrate at low concentrations, and decreased the proteolytic activity of Candida albicans at mini inhibitory concentration. Finally, the EO and its selected active fraction had low cytotoxicity on human cells, mechanisms affecting gene expression in pathways involving chernokines and MAP-kinase (proliferation/apoptosis), as adhesion proteins. These findings highlight the potential antifungal activity of the EO from C. sativum leaves and suggest avenues for future translational toxicological research. (AU)

Processo FAPESP: 11/15984-0 - Avaliação da atividade anti-Candida, antiproliferativa e citotóxica do óleo essencial e frações de Coriandrum sativum L.
Beneficiário:Irlan de Almeida Freires
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 11/14757-0 - Avaliação da atividade antimicrobiana de óleos essenciais sobre patógenos bucais
Beneficiário:Pedro Luiz Rosalen
Linha de fomento: Auxílio à Pesquisa - Regular