Photobiological effect of low-level laser irradiation in bovine embryo production system

Autor(es)

Soares, Carlos Alexandre ^[1] ; Annes, Kelly ^[1] ; Dreyer, Thiago Revers ^[1] ; Magrini, Taciana ^[1] ; Sonoda, Mayra Trentin ^[1] ; Martinho, Herculano da Silva ^[1] ; Nichi, Marcilio ^[2] ; Ortiz d'Avila Assumpcao, Mayra Elena ^[2] ; Milazzotto, Marcella Pecora ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed ABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil

^[2] Univ Sao Paulo, Dept Anim Reprod, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Optics; v. 19, n. 3 MAR 2014.
• Citações Web of Science: 10

Resumo

The objectives of this study were to evaluate the effect of low-level laser irradiation (LLLI) on bovine oocyte and granulosa cells metabolism during in vitro maturation (IVM) and further embryo development. Cumulus-oocytes complexes (COCs) were subjected (experimental group) or not (control group) to irradiation with LLLI in a 633-nm wavelength and 1 J/cm(2) fluency. The COCs were evaluated after 30 min, 8, 16, and 24 h of IVM. Cumulus cells were evaluated for cell cycle status, mitochondrial activity, and viability (flow cytometry). Oocytes were assessed for meiotic progression status (nuclear staining), cell cycle genes content {[}real-time polymerase chain reaction (PCR)], and signal transduction status (western blot). The COCs were also in vitro fertilized, and the cleavage and blastocyst rates were assessed. Comparisons among groups were statistically performed with 5% significance level. For cumulus cells, a significant increase in mitochondrial membrane potential and the number of cells progressing through the cycle could be observed. Significant increases on cyclin B and cyclin-dependent kinase (CDK4) levels were also observed. Concerning the oocytes, a significantly higher amount of total mitogen-activated protein kinase was found after 8 h of irradiation, followed by a decrease in all cell cycle genes transcripts, exception made for the CDK4. However, no differences were observed in meiotic progression or embryo production. In conclusion, LLLI is an efficient tool to modulate the granulosa cells and oocyte metabolism. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE) (AU)

• Processo FAPESP: 09/51630-9 - Efeito fotobiologico do laser de baixa intensidade em gametas e embrioes bovinos
• Beneficiário: Marcella Pecora Milazzotto
• Modalidade de apoio: Auxílio à Pesquisa - Regular