Sugar-Mediated Disassembly of Mucin/Lectin Multi layers and Their Use as pH-Tolerant, On-Demand Sacrificial Layers

Autor(es)

Polak, Roberta ^[1] ; Crouzier, Thomas ^[2] ; Lim, Rosanna M. ^[3] ; Ribbeck, Katharina ^[2] ; Beppu, Marisa M. ^[4] ; Pitombo, Ronaldo N. M. ^[1] ; Cohen, Robert E. ^[3] ; Rubner, Michael F. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508000 Sao Paulo - Brazil

^[2] MIT, Dept Biol Engn, Cambridge, MA 02139 - USA

^[3] MIT, Dept Chem Engn, Cambridge, MA 02139 - USA

^[4] Univ Estadual Campinas, UNICAMP, Sch Chem Engn, BR-13083852 Campinas, SP - Brazil

^[5] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 - USA

• Tipo de documento: Artigo Científico
• Fonte: Biomacromolecules; v. 15, n. 8, p. 3093-3098, AUG 2014.
• Citações Web of Science: 19

Resumo

The layer-by-layer (LbL) assembly of thin films on surfaces has proven to be an extremely useful technology for uses ranging from optics to biomedical applications. Releasing these films from the substrate to generate so-called free-standing multilayer films opens a new set of applications. Current approaches to generating such materials are limited because they can be cytotoxic, difficult to scale up, or have undesirable side reactions on the material. In this work, a new sacrificial thin film system capable of chemically triggered dissolution at physiological pH of 7.4 is described. The film was created through LbL assembly of bovine submaxillary mucin (BSM) and the lectin jacalin (JAC) for a (BSM/JAC) multilayer system, which remains stable over a wide pH range (pH 3-9) and at high ionic strength (up to 5 M NaCl). This stability allows for subsequent LbL assembly of additional films in a variety of conditions, which could be released from the substrate by incubation in the presence of a competitive inhibitor sugar, melibiose, which selectively disassembles the (BSM/JAC) section of the film. This novel multilayer system was then applied to generate free-standing, 7 mu m diameter, circular ultrathin films, which can be attached to a cell surface as a ``backpack{''}. A critical thickness of about 100 nm for the (BSM/JAC) film was required to release the backpacks from the glass substrate, after incubation in melibiose solution at 37 degrees C for 1 h. Upon their release, backpacks were subsequently attached to murine monocytes without cytotoxicity, thereby demonstrating the compatibility of this mucin-based release system with living cells. (AU)

• Processo FAPESP: 10/51066-3 - Desenvolvimento e fabricacao de filmes ultra-finos, obtidos pela tecnica layer-by-layer, para aplicacoes na entrega direcionada de farmacos e na captura seletiva de bio-marcadores.
• Beneficiário: Roberta Polak
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/21326-6 - Desenvolvimento de filme funcional via técnica de layer-by-layer para liberação de droga antineoplásica encapsulada em lipossomas ecogênicos
• Beneficiário: Roberta Polak
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado