VIII Workshop de Proteômica

• Processo: 18/09315-8
• Modalidade de apoio: Auxílio Organização - Reunião Científica
• Data de Início da vigência: 05 de novembro de 2018
• Data de Término da vigência: 08 de novembro de 2018
• Área do conhecimento: Ciências da Saúde - Medicina
• Pesquisador responsável: Adriana Franco Paes Leme
• Beneficiário: Adriana Franco Paes Leme
• Instituição Sede: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brasil
• Assunto(s): Espectrometria de massas Proteômica
• Palavra(s)-Chave do Pesquisador: proteômica | Espectrometria de Massas

Resumo

Theoretical and Hands-on ProgramDay 18:30 AMWelcome - Adriana Paes Leme 9:00 AMIntroduction to quantitative analysis - Tina Ludwig 10:30 AMCoffee break11:00 AMBasics of targeted mass spec (relative/absolute Quant) - Tina Ludwig12:00 PMLunch 1:00 PMBiological relevance of targeted proteomics (case study) - Ariel Bensimon2:00 PMIntroduction to Skyline - Ariel Bensimon 2:30 PMTutorial: SRM method development - Tina Ludwig/ Ariel Bensimon4:00 PMCoffee break 4:30 PMTutorial: Data analysis - Tina Ludwig/ Ariel Bensimon 6:00 PMScience Slam Day 29:00 AMBuilding prior knowledge for targeted proteomics - Birgit Schilling10:15 AMCoffee break 10:45 AMHands-On: Method refinement - Brendan MacLean (online)12:00 PMLunch 1:00 PMSystem suitability and QC - Birgit Schilling2:30 PMHands-On: Panorama targeted knowledge base - Birgit Schilling4:00 PMCoffee break 4:00 PMHands-On: Grouped study data processing with Skyline - Ariel BensimonDay 39:00 AMHands on retention time prediction with iRT in Skyline - Lindsay Pino10:15 AMCoffee break 10:45 AMStudy design and statistical considerations - Lindsay Pino12:00 PMLunch 1:00 PMMSstats and its integration with Skyline - Lindsay Pino1:30 PMTutorial: MSstats for statistical analysis - Lindsay Pino2:30 PMTargeting proteomics at high throughput (research examples) - Lindsay Pino3:30 PMCoffee break4:00 PMMS1 Filtering for PTM studies - Birgit Schilling5:00 PMHands-On: MS1 Filtering with Skyline - Birgit SchillingDay 49:00 AMFrom DDA to PRM - Birgit Schilling10:00 AMCoffee break10:30 AMHands-On: PRM with Skyline - Birgit Schilling11:30 AMIntroduction to Data-Independent Acquisition (DIA) - Lindsay Pino12:00 PMLunch1:00 PMHands-On: Analysis of DIA data in Skyline - Lindsay Pino2:30 PMLibrary Peak Picking with mProphet - Lindsay Pino3:30 PMCoffee break 4:00 PM Future of targeted proteomics - Birgit Schilling4:30 PMFeedback and Cou (AU)