Nitric oxide levels in cultured chinese hamster lung fibroblasts transfected with the human gene for cu/zn superoxido dismutase

• Processo: 94/03778-0
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Data de Início da vigência: 01 de janeiro de 1995
• Data de Término da vigência: 29 de fevereiro de 1996
• Área do conhecimento: Ciências Biológicas - Farmacologia - Farmacologia Bioquímica e Molecular
• Pesquisador responsável: Stephen Hyslop
• Beneficiário: Stephen Hyslop
• Instituição Sede: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brasil
• Assunto(s): Óxido nítrico Fibroblastos Superóxido dismutase Guanilato ciclase
• Palavra(s)-Chave do Pesquisador: Cyclic Gmp | Fibroblasts | Guanylate Cyclase | Nitric Oxide | Superoxide Anion | Superoxido Dismutase

Resumo

In this study, we propose to investigate the importance of intracellular superoxide dismutase (SOD) in regulating the concentrations of NO (Nitric Oxide) by comparing the amounts of Guanosine Cyclic 3':5'-Monophosphate (cGMP) formed by the enzyme soluble Guanylate Cyclase in normal V79 Chinese Hamster lung fibroblasts with those of transfected V79 cells which over express the inserted human gene for Cu/Zn sod. The results of this study should provide some insight into the importance of sod in regulating intracellular NO quantities and may also be of potential therapeutic importance in promoting the development of agents capable of modulating the concentrations of sod in vivo, a means of controlling NO levels. (AU)