Biossensores baseados em nanoestruturas peptídicas funcionalizadas para detecção e diagnóstico de doenças

• Processo: 12/24729-7
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Data de Início da vigência: 01 de julho de 2013
• Data de Término da vigência: 30 de junho de 2015
• Área de conhecimento: Ciências Exatas e da Terra - Química - Físico-química
• Pesquisador responsável: Wendel Andrade Alves
• Beneficiário: John Jairo Castillo León
• Instituição Sede: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brasil
• Assunto(s): Eletroquímica Biomateriais Peptídeos Diagnóstico bucal
• Palavra(s)-Chave do Pesquisador: Biomateriais | biossensores | Eletroquímica | Nanoestruturas de peptídeos | química supramolecular | Eletroquímica

Resumo

Peptide nanostructures are biological entities that are able to self-organize under mild conditions rapidly and without employing specialized equipment or high temperatures. Modification of the peptide nanostructures with biomolecules can increase their properties for various nanotechnological applications. The objective of this research proposal is to describe the preparation and functionalization of peptide nanostructures for construction of a biosensor able to detect cells over-expressing folate receptors responsible of diseases such cancer and leishmaniasis. The functionalized peptide nanostructures will be characterized by ultraviolet-visible, fluorescence, nuclear magnetic resonance and Raman spectroscopies. While the size and structural details will be analyze using scanning and transmission electron microscopies, atomic force microscopy and fluorescence microscopy. The biosensor will be design through the integration of the functionalized peptide nanostructures in graphene and carbon electrodes. Immobilization and modification of the electrodes will be evaluated by means of electrochemical impedance, amperometry and cyclic voltammetry techniques. Parameters such as selectivity, stability and detection limit will be useful to evaluate the performance of the biosensor. The biosensor based on modified peptide nanostructures obtained in this research will be applied in early stage diagnoses of cancer disease and in the detection of cells infected with the parasite producing leishmaniasis. (AU)