Expression of the plant uncoupling mitochondrial protein (pump) in yeast.

• Processo: 98/00565-6
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Data de Início da vigência: 01 de setembro de 1998
• Data de Término da vigência: 31 de agosto de 2000
• Área de conhecimento: Ciências Biológicas - Genética - Genética Vegetal
• Pesquisador responsável: Jiri Borecky
• Beneficiário: Jiri Borecky
• Instituição Sede: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brasil
• Vinculado ao auxílio: 94/03581-1 - Mecanismos de transporte e metabolismo energético em sistema biológico, AP.TEM
• Assunto(s): Arabidopsis thaliana Mitocôndrias
• Palavra(s)-Chave do Pesquisador: Arabidopsis Thaliana | Bioenegetics | Mitochondria | Plant Uncoupling Protein | Pump | Tomato

Resumo

Plant uncoupling mitochondrial protein (PUMP) has been identified by Vercesi and in cooperation with Dr. Petr Jezek, from the same home laboratory as the applicant, have been further characterized in potato tuber and tomato mitochondria. It was found that PUMP is able to transport anionic fatty acids and alkyl sulfonates but not chloride or pyruvate and that transport was inhibited by ATP or GDP, similarly as the mammalian uncoupling protein (UCP1). In the laboratory of Dr. Paulo Arruda, cDNA clones encoding PUMP were isolated and are currently being characterized. The full lenght cDNA clones will be expressed in yeast. (AU)