| Texto completo | |
| Autor(es): |
Santos, Anselmo Azevedo
[1]
;
Penha, Helen Alves
[1]
;
Bellec, Arnaud
[2]
;
Munhoz, Carla de Freitas
[1]
;
Pedrosa-Harand, Andrea
[3]
;
Berges, Helene
[2]
;
Carneiro Vieira, Maria Lucia
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Piracicaba - Brazil
[2] INRA, Ctr Natl Ressources Genom Vegetales, F-31326 Castanet Tolosan - France
[3] Univ Fed Pernambuco, Dept Bot, BR-50670420 Recife, PE - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BMC Genomics; v. 15, SEP 26 2014. |
| Citações Web of Science: | 14 |
| Resumo | |
Background: The passion fruit (Passiflora edulis) is a tropical crop of economic importance both for juice production and consumption as fresh fruit. The juice is also used in concentrate blends that are consumed worldwide. However, very little is known about the genome of the species. Therefore, improving our understanding of passion fruit genomics is essential and to some degree a pre-requisite if its genetic resources are to be used more efficiently. In this study, we have constructed a large-insert BAC library and provided the first view on the structure and content of the passion fruit genome, using BAC-end sequence (BES) data as a major resource. Results: The library consisted of 82,944 clones and its levels of organellar DNA were very low. The library represents six haploid genome equivalents, and the average insert size was 108 kb. To check its utility for gene isolation, successful macroarray screening experiments were carried out with probes complementary to eight Passiflora gene sequences available in public databases. BACs harbouring those genes were used in fluorescent in situ hybridizations and unique signals were detected for four BACs in three chromosomes (n = 9). Then, we explored 10,000 BES and we identified reads likely to contain repetitive mobile elements (19.6% of all BES), simple sequence repeats and putative proteins, and to estimate the GC content (similar to 42%) of the reads. Around 9.6% of all BES were found to have high levels of similarity to plant genes and ontological terms were assigned to more than half of the sequences analysed (940). The vast majority of the top-hits made by our sequences were to Populus trichocarpa (24.8% of the total occurrences), Theobroma cacao (21.6%), Ricinus communis (14.3%), Vitis vinifera (6.5%) and Prunus persica (3.8%). Conclusions: We generated the first large-insert library for a member of Passifloraceae. This BAC library provides a new resource for genetic and genomic studies, as well as it represents a valuable tool for future whole genome study. Remarkably, a number of BAC-end pair sequences could be mapped to intervals of the sequenced Arabidopsis thaliana, V. vinifera and P. trichocarpa chromosomes, and putative collinear microsyntenic regions were identified. (AU) | |
| Processo FAPESP: | 11/51823-1 - Construcao e exploracao de uma biblioteca genomica de passiflora edulis em bacs (bacterial artificial chromosomes). |
| Beneficiário: | Maria Lúcia Carneiro Vieira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |