Expression of receptors of advanced glycation end product (RAGE) and types I, III and IV collagen in the vastus lateralis muscle of men in early stages of knee osteoarthritis

Autor(es)

Serrao, Paula Regina M. S. ^[1] ; Vasilceac, Fernando A. ^[2] ; Gramani-Say, Karina ^[2] ; Lessi, Giovanna C. ^[1] ; Reiff, Rodrigo B. M. ^[3] ; Mattiello-Sverzut, Ana Claudia ^[4] ; Mattiello, Stela M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Phys Therapy, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Gerontol, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Med, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Dept Biomech Med & Rehabil Locomotor Apparatus, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Connective Tissue Research; v. 55, n. 5-6, p. 331-338, OCT-DEC 2014.
• Citações Web of Science: 1

Resumo

Alterations in the contractile and non-contractile proteins of the skeletal muscle may reduce muscle function in knee osteoarthritis (OA), and the formation and accumulation of advanced glycation end products, particularly in collagen, can influence the quality of these muscle proteins. The objective of this study was to evaluate the reactivity of types I, III and IV collagen and the expression and localization of receptor for advanced glycation end products (RAGE) in the vastus lateralis (VL) muscle in early stages of knee OA. The hypothesis was that these patients present a higher expression of RAGE and increased immunoreactivity in the collagen. Thirty-five men were divided into two groups: the control group (CG; n = 17) and the osteoarthritis group (OAG; n = 18). All participants were submitted to a biopsy of the VL. The muscle samples were analyzed by immunohistochemistry for collagen and for RAGE and laminin. The expression of RAGE was counted (intracellular, extracellular and total). Student's t-test for independent samples and Mann-Whitney U test were used for the RAGE's intergroup analysis (alpha <= 0.05). A semiquantitative analysis was performed to assess the collagen reactivity. No significant differences were observed in the intracellular, extracellular or total localization of RAGE (p > 0.05). Higher immunoreactivity was observed in the OAG for all types of collagen, with more reactivity for collagen III and IV. We concluded that in the initial stages of knee OA, no differences were observed for RAGE levels between the groups. However, the OAG's higher collagen expression may represent adaptations for reducing muscle stiffness and avoiding injury. (AU)

• Processo FAPESP: 07/07200-4 - Aspectos funcionais, morfológicos e imuno-histoquímicos do músculo quadríceps femoral de indivíduos com graus I ou II de Osteoartrite de joelho
• Beneficiário: Paula Regina Mendes da Silva Serrão
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 11/06619-7 - Influência das alterações de sono no controle sensório-motor e na resposta inflamatória sistêmica em pacientes com osteoartrite de joelho graus I ou II
• Beneficiário: Stela Márcia Mattiello
• Modalidade de apoio: Auxílio à Pesquisa - Regular