Expression of receptors of advanced glycation end product (RAGE) and types I, III and IV collagen in the vastus lateralis muscle of men in early stages of knee osteoarthritis

Author(s)

Serrao, Paula Regina M. S. ^[1] ; Vasilceac, Fernando A. ^[2] ; Gramani-Say, Karina ^[2] ; Lessi, Giovanna C. ^[1] ; Reiff, Rodrigo B. M. ^[3] ; Mattiello-Sverzut, Ana Claudia ^[4] ; Mattiello, Stela M. ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Phys Therapy, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Gerontol, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Med, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Dept Biomech Med & Rehabil Locomotor Apparatus, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Connective Tissue Research; v. 55, n. 5-6, p. 331-338, OCT-DEC 2014.
• Web of Science Citations: 1

Abstract

Alterations in the contractile and non-contractile proteins of the skeletal muscle may reduce muscle function in knee osteoarthritis (OA), and the formation and accumulation of advanced glycation end products, particularly in collagen, can influence the quality of these muscle proteins. The objective of this study was to evaluate the reactivity of types I, III and IV collagen and the expression and localization of receptor for advanced glycation end products (RAGE) in the vastus lateralis (VL) muscle in early stages of knee OA. The hypothesis was that these patients present a higher expression of RAGE and increased immunoreactivity in the collagen. Thirty-five men were divided into two groups: the control group (CG; n = 17) and the osteoarthritis group (OAG; n = 18). All participants were submitted to a biopsy of the VL. The muscle samples were analyzed by immunohistochemistry for collagen and for RAGE and laminin. The expression of RAGE was counted (intracellular, extracellular and total). Student's t-test for independent samples and Mann-Whitney U test were used for the RAGE's intergroup analysis (alpha <= 0.05). A semiquantitative analysis was performed to assess the collagen reactivity. No significant differences were observed in the intracellular, extracellular or total localization of RAGE (p > 0.05). Higher immunoreactivity was observed in the OAG for all types of collagen, with more reactivity for collagen III and IV. We concluded that in the initial stages of knee OA, no differences were observed for RAGE levels between the groups. However, the OAG's higher collagen expression may represent adaptations for reducing muscle stiffness and avoiding injury. (AU)

• FAPESP's process: 07/07200-4 - Functional, morphological and immunohistochemical aspects of the quadriceps muscle in subjects with knee Osteoarthritis grade I or II
• Grantee: Paula Regina Mendes da Silva Serrão
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 11/06619-7 - Influence of changes of sleep in sensory-motor control and systemic inflammatory response in patients with knee osteoarthritis of grade I or II
• Grantee: Stela Márcia Mattiello
• Support Opportunities: Regular Research Grants