Characterization and spray drying of lipase produced by the endophytic fungus Cercospora kikuchii

Autor(es)

Costa-Silva, T. A. ^[1] ; Souza, C. R. F. ^[1] ; Oliveira, W. P. ^[1] ; Said, S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14040903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Chemical Engineering; v. 31, n. 4, p. 849-858, OCT-DEC 2014.
• Citações Web of Science: 8

Resumo

A lipase from the endophytic fungus Cercospora kikuchii was purified, biochemically characterized and the effects of spray drying on stabilization of the purified enzyme were studied. The lipase was purified 9.31-fold with recovery of 26.6% and specific activity of 223.6 U/mg. The optimum pH and temperature were 4.6 and 35 ºC, respectively, while the Vmax was 10.28 µmol/min.mg-1 protein and Km 0.0324 mM. All the metal ions tested enhanced the enzyme activity. The lipase retained almost 100% activity in the presence of strong oxidants and was also resistant to Triton X, Tween 80 and 20 and SDS, as well as to proteases. The purified lipase was spray dried and kept until 85.2% of enzymatic activity. At least 70% of the enzymatic activity was maintained for spray dried purified lipase during the storage period. The lipase produced by Cercospora kikuchii has properties useful for industrial application and showed adequate stabilization and retention of its enzymatic activity after spray drying. (AU)

• Processo FAPESP: 04/07935-6 - Bioprospecção de fungos: a busca de compostos importantes para o projeto de remédios e enzimas para aplicações farmacêuticas e industriais
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 08/52732-7 - Caracterização bioquímica e secagem por spray-dryer de lipases produzidas pelo fungo endofítico Cercospora kikuchii
• Beneficiário: Tales Alexandre da Costa e Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado