Towards a phylogenetic position for the morphologically-defined genus Pannus (Cyanobacteria)

Autor(es)

da Silva Malone, Camila Francieli ^[1] ; Alvarenga, Danillo Oliveira ^[2] ; Fiore, Marli Fatima ^[2] ; Sant'Anna, Celia Leite ^[1]

Afiliação do(s) autor(es)

^[1] Inst Bot, BR-04301012 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13400970 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NOVA HEDWIGIA; v. 99, n. 3-4, p. 511-524, 2014.
• Citações Web of Science: 7

Resumo

The morphologically-defined genus Pannus containing members of unicellular cyanobacteria, has been variously classified belonging to the families Microcystaceae, Merismopediaceae, and Synechococcaceae. The phylogeny of Pannus, which possesses cells disposed in one layer at the mucilage surface, two planes of cell division and no gas vesicles, has yet to be developed. In this study, in a combined approach of morphological and ultrastructural analyses, 16S rRNA phylogeny and comparison of the ITS nucleotide sequence and secondary structure, the status of a Pannus strain isolated from a Brazilian wetland area was examined. Morphologically, the new strain CCIBt3594 showed all the diagnostic traits of the genus Pannus according to the original concept. Phylogenetic analysis of 16S rDNA sequence indicated that strain CCIBt3594 is affiliated to the genus Microcystis, but with low similarity (< 94.4%), confirming that this strain indeed belongs to a distinct genus. The evaluation of 16S-23S ITS nucleotide sequence and secondary structure, as well as the disposition of the thylakoid structures, also indicated that strain CCIBt3594 resembles Microcystis. Therefore, the molecular and ultrastructural data obtained in this study corroborate with the initial findings that the genus Pannus belongs to the order Chroococcales and most likely to the family Microcystaceae. This Brazilian strain differs from the already known humus species by a larger cell size, absence of individual mucilage and its habitat, and is here described for the first time as Pannus brasiliensis sp. nov. (AU)

• Processo FAPESP: 11/08092-6 - Sequenciamento do genoma da cianobactéria Nostoc sp. CENA67 e anotação de genes do metabolismo secundário
• Beneficiário: Danillo Oliveira de Alvarenga
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/51666-0 - Diversidade fenotipica, molecular e avaliacao do potencial biotecnologico do genero phormidium (cyanobacteria) em ambientes aquaticos e aerofiticos da mata atlantica e pantanal, brasil
• Beneficiário: Camila Francieli da Silva Malone
• Modalidade de apoio: Bolsas no Brasil - Doutorado