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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Towards a phylogenetic position for the morphologically-defined genus Pannus (Cyanobacteria)

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Author(s):
da Silva Malone, Camila Francieli [1] ; Alvarenga, Danillo Oliveira [2] ; Fiore, Marli Fatima [2] ; Sant'Anna, Celia Leite [1]
Total Authors: 4
Affiliation:
[1] Inst Bot, BR-04301012 Sao Paulo - Brazil
[2] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13400970 Piracicaba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: NOVA HEDWIGIA; v. 99, n. 3-4, p. 511-524, 2014.
Web of Science Citations: 7
Abstract

The morphologically-defined genus Pannus containing members of unicellular cyanobacteria, has been variously classified belonging to the families Microcystaceae, Merismopediaceae, and Synechococcaceae. The phylogeny of Pannus, which possesses cells disposed in one layer at the mucilage surface, two planes of cell division and no gas vesicles, has yet to be developed. In this study, in a combined approach of morphological and ultrastructural analyses, 16S rRNA phylogeny and comparison of the ITS nucleotide sequence and secondary structure, the status of a Pannus strain isolated from a Brazilian wetland area was examined. Morphologically, the new strain CCIBt3594 showed all the diagnostic traits of the genus Pannus according to the original concept. Phylogenetic analysis of 16S rDNA sequence indicated that strain CCIBt3594 is affiliated to the genus Microcystis, but with low similarity (< 94.4%), confirming that this strain indeed belongs to a distinct genus. The evaluation of 16S-23S ITS nucleotide sequence and secondary structure, as well as the disposition of the thylakoid structures, also indicated that strain CCIBt3594 resembles Microcystis. Therefore, the molecular and ultrastructural data obtained in this study corroborate with the initial findings that the genus Pannus belongs to the order Chroococcales and most likely to the family Microcystaceae. This Brazilian strain differs from the already known humus species by a larger cell size, absence of individual mucilage and its habitat, and is here described for the first time as Pannus brasiliensis sp. nov. (AU)

FAPESP's process: 11/08092-6 - Genome sequencing of the cyanobacterium Nostoc sp. CENA67 and anotation of secondary metabolism genes
Grantee:Danillo Oliveira de Alvarenga
Support Opportunities: Scholarships in Brazil - Doctorate