Fabrication of Microenvironments with Different Geometrical Features for Cell Growth Studies

Autor(es)

Avila, O. I. ^[1] ; Otuka, A. J. G. ^[1] ; Tribuzi, V. ^[1] ; Freitas, L. M. ^[2] ; Serafim, R. B. ^[2] ; Moraes, M. H. ^[3] ; Espreafico, E. M. ^[3] ; Valente, V. ^{[2, 4]} ; Fontana, C. R. ^[2] ; Mendonca, C. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Fac Ciencias Farmaceut, BR-14801960 Araraquara, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] NAP USP, CISBi Ctr Integrat Syst Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF LASER MICRO NANOENGINEERING; v. 9, n. 3, p. 248-251, NOV 2014.
• Citações Web of Science: 1

Resumo

Studies of cell development in artificial microenvironments can contribute to understanding a series of physiological mechanisms that might be influenced by geometrical features of the microenvironment itself. In this work we applied two-photon polymerization to fabricate three-dimensional microenvironments composed of a matrix arrangement of microstructures (circular and square cross-sections pillars) with distinct spacing. Such microenvironments were used to evaluate the growth of Michigan Cancer Foundation-7 (MCF-7) cells that are commonly used as a model system to investigate fundamental aspects of the tumor biology. Our results reveal that the cell density decreases as the distance between structures in the environment is increased. Additionally, cell growth shows slightly better results for the microenvironments composed of circular cross-section structures. (AU)

• Processo FAPESP: 11/12399-0 - Aplicações de pulsos de femtossegundos em óptica não linear: espectroscopia, formatação de pulsos e microfabricação
• Beneficiário: Cleber Renato Mendonça
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/21807-7 - Fabricação via fotopolimerização por absorção de dois fótons de cristais fotônicos em polímeros dopados
• Beneficiário: Vinicius Tribuzi Rodrigues Pinheiro Gomes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado