Molecular Genetic Variability of Commercial and Wild Accessions of Passion Fruit (Passiflora spp.) Targeting ex Situ Conservation and Breeding

Autor(es)

Cerqueira-Silva, Carlos Bernard M. ^{[1, 2]} ; Santos, Elisa S. L. ^{[2, 1]} ; Jesus, Onildo N. ^[3] ; Vieira, Joao G. P. ^[1] ; Mori, Gustavo M. ^[1] ; Correa, Ronan X. ^[4] ; Souza, Anete P. ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Mol Biol & Genet Engn Ctr, BR-13083875 Campinas, SP - Brazil

^[2] State Univ Southwest Bahia, Lab Appl Mol Genet, Dept Exact & Nat Sci, BR-45700000 Itapetinga - Brazil

^[3] Brazilian Agr Res Corp, Cassava & Fruits, BR-44380000 Cruz Das Almas - Brazil

^[4] Univ Estadual Santa Cruz, Biotechnol & Genet Ctr, Dept Biol Sci, BR-45662900 Ilheus - Brazil

^[5] Univ Estadual Campinas, Dept Plant Biol, Inst Biol, BR-13083875 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 15, n. 12, p. 22933-22959, DEC 2014.
• Citações Web of Science: 4

Resumo

Passiflora species are distributed throughout Latin America, and Brazil and Colombia serve as the centers of diversity for this genus. We performed cross-species amplification to evaluate 109 microsatellite loci in 14 Passiflora species and estimated the diversity and genetic structure of Passiflora cincinnata, Passiflora setaceae and Passiflora edulis. A total of 127 accessions, including 85 accessions of P. edulis, a commercial species, and 42 accessions of 13 wild species, were examined. The cross-species amplification was effective for obtaining microsatellite loci (average cross-amplification of 70%). The average number of alleles per locus (five) was relatively low, and the average diversity ranged from 0.52 in P. cincinnata to 0.32 in P. setacea. The Bayesian analyses indicated that the P. cincinnata and P. setacea accessions were distributed into two groups, and the P. edulis accessions were distributed into five groups. Private alleles were identified, and suggestions for core collections are presented. Further collections are necessary, and the information generated may be useful for breeding and conservation. (AU)

• Processo FAPESP: 08/52197-4 - Genomic-assisted breeding of sugarcane: using molecular markers for understanding the genetic architecture of quantitative traits and to implement marker assisted selection
• Beneficiário: Anete Pereira de Souza
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático