Intrinsically disordered proteins (IDPs) in trypanosomatids

Autor(es)

Ruy, Patrcia de Cassia ^[1] ; Torrieri, Raul ^[1] ; Toledo, Juliano Simoes ^[2] ; Alves, Viviane de Souza ^[3] ; Cruz, Angela Kaysel ^[2] ; Ruiz, Jeronimo Conceicao ^[1]

Afiliação do(s) autor(es)

^[1] Fiocruz MS, Fdn Oswaldo Cruz, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

^[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Genomics; v. 15, DEC 13 2014.
• Citações Web of Science: 6

Resumo

Background: Proteins are composed of one or more amino acid chains and exhibit several structure levels. IDPs (intrinsically disordered proteins) represent a class of proteins that do not fold into any particular conformation and exist as dynamic ensembles in their native state. Due to their intrinsic adaptability, IDPs participate in many regulatory biological processes, including parasite immune escape. Using the information from trypanosomatids proteomes, we developed a pipeline for the identification, characterization and analysis of IDPs. The pipeline employs six disorder prediction methodologies and integrates structural and functional annotation information, subcellular location prediction and physicochemical properties. At the core of the IDP pipeline, there is a relational database that describes the protein disorder knowledge in a logically consistent manner. Results: The results obtained from the IDP pipeline showed that Leishmania and Trypanosoma species have approximately 70% and 55% IDPs, respectively. Our results indicate that IDPs in trypanosomatids contain disorder-promoting amino acids and order-promoting amino acids. The functional annotation analysis demonstrated enrichment of selected Gene Ontology terms. A relevant association was observed between the disordered residue numbers within predicted IDPs and their subcellular location, lack of transmembrane domains and lack of predicted function. We validated our computational findings with 2D electrophoresis designed for IDP identification and found that 100% of the identified protein spots were predicted in silico. Conclusions: Because there is no pipeline or database addressing IDPs in trypanosomatids, the pipeline described here represents the first attempt to establish possible correlations between protein function and structural disorder in these eukaryotes. Interestingly, all significant associations detected in the contingency analysis were observed when the protein disorder content reached approximately 40%. The exploratory data analysis allowed us to develop hypotheses regarding the IDPs' association with key biological features of these parasites, including transcription and transcriptional regulation, RNA processing and splicing, and cytoskeleton. (AU)

• Processo FAPESP: 06/50323-7 - Estudo do controle da expressão gênica e plasticidade genética em Leishmania
• Beneficiário: Angela Kaysel Cruz
• Modalidade de apoio: Auxílio à Pesquisa - Temático