Follicular fluid lipid fingerprinting from women with PCOS and hyper response during IVF treatment

Autor(es)

Cordeiro, Fernanda Bertuccez ^[1] ; Cataldi, Thais Regiani ^[1] ; Teixeira da Costa, Livia do Vale ^[1] ; de Lima, Camila Bruna ^[1] ; Stevanato, Juliana ^[1] ; Zylbersztejn, Daniel Suslik ^[1] ; Ferreira, Christina Ramires ^[2] ; Eberlin, Marcos Nogueira ^[2] ; Cedenho, Agnaldo Pereira ^[1] ; Lo Turco, Edson Guimaraes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Human Reprod Sect, Dept Surg, Div Urol, BR-04039060 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ASSISTED REPRODUCTION AND GENETICS; v. 32, n. 1, p. 45-54, JAN 2015.
• Citações Web of Science: 7

Resumo

Polycystic ovary syndrome (PCOS) is an endocrine-metabolic disorder that leads to lower natural reproductive potential and presents a challenge for assisted reproductive medicine because patients may exhibit immature oocyte retrieval and a higher risk of ovarian hyper stimulation syndrome during in vitro fertilization (IVF) treatment. This study aimed to identify potential lipid biomarkers for women with PCOS and a hyper response to controlled ovarian stimulation. Follicular fluid samples were collected from patients who underwent IVF, including normal responder women who became pregnant (control group, n = 11), women with PCOS and a hyper response to gonadotropins (PCOS group, n = 7) and women with only hyper response to gonadotropins (HR group, n = 7). A lipidomic analysis was performed by electrospray ionization mass spectrometry, and candidate biomarkers were analyzed by tandem mass spectrometry experiment. The lipid profiles indicated particularities related to differences in phosphatidylcholine (PCOS and HR), phosphatidylserine, phosphatydilinositol and phosphatidylglycerol (control), sphingolipids (PCOS) and phosphatidylethanolamine (control and HR). These findings contribute to the understanding of the molecular mechanisms associated with lipid metabolism in the PCOS-related hyper response, and strongly suggest that these lipids may be useful as biomarkers, leading to the development of more individualized treatment for pregnancy outcome. (AU)

• Processo FAPESP: 12/06389-4 - Determinação do perfil lipídico do líquido folicular e plasma sanguíneo de mulheres com e sem síndrome dos ovários policísticos submetidas ao tratamento de fertilização in vitro
• Beneficiário: Edson Guimarães Lo Turco
• Modalidade de apoio: Auxílio à Pesquisa - Regular