Global Biogeographic Sampling of Bacterial Secondary Metabolism

Autor(es)

Charlop-Powers, Zachary ^[1] ; Owen, Jeremy G. ^[1] ; Reddy, Boojala Vijay B. ^[1] ; Ternei, Melinda ^[1] ; Guimaraes, Denise O. ^[2] ; de Frias, Ulysses A. ^[3] ; Pupo, Monica T. ^[3] ; Seepe, Prudy ^[4] ; Feng, Zhiyang ^[5] ; Brady, Sean F. ^[1]

Afiliação do(s) autor(es)

^[1] Rockefeller Univ, Lab Genet Encoded Small Mol, Howard Hughes Med Inst, New York, NY 10065 - USA

^[2] Univ Fed Rio de Janeiro, Lab Prod Nat, Curso Farm, BR-27933378 Macae, RJ - Brazil

^[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, FCFRP USP, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] K RITH KwaZulu Natal Res Inst TB & HIV, Nelson R Mandela Sch Med, Durban - South Africa

^[5] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu - Peoples R China

• Tipo de documento: Artigo Científico
• Fonte: eLIFE; v. 4, JAN 19 2015.
• Citações Web of Science: 40

Resumo

Recent bacterial (meta) genome sequencing efforts suggest the existence of an enormous untapped reservoir of natural-product-encoding biosynthetic gene clusters in the environment. Here we use the pyrosequencing of PCR amplicons derived from both nonribosomal peptide adenylation domains and polyketide ketosynthase domains to compare biosynthetic diversity in soil microbiomes from around the globe. We see large differences in domain populations from all except the most proximal and biome-similar samples, suggesting that most microbiomes will encode largely distinct collections of bacterial secondary metabolites. Our data indicate a correlation between two factors, geographic distance and biome-type, and the biosynthetic diversity found in soil environments. By assigning reads to known gene clusters we identify hotspots of biomedically relevant biosynthetic diversity. These observations not only provide new insights into the natural world, they also provide a road map for guiding future natural products discovery efforts. (AU)

• Processo FAPESP: 11/50869-8 - Utilização da metagenômica como ferramenta para a busca de novos produtos naturais microbianos
• Beneficiário: Mônica Tallarico Pupo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular