Synthesis and characterization of robust magnetic carriers for bioprocess applications

Autor(es)

Kopp, Willian ^[1] ; Silva, Felipe A. ^[1] ; Lima, Lionete N. ^[1] ; Masunaga, Sueli H. ^[2] ; Tardioli, Paulo W. ^[3] ; Giordano, Roberto C. ^[3] ; Araujo-Moreira, Fernando M. ^[4] ; Giordano, Raquel L. C. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos UFScar, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

^[2] Montana State Univ, Dept Phys, Bozeman, MT 59717 - USA

^[3] Univ Fed Sao Carlos UFScar, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Fed Sao Carlos UFScar, Dept Phys, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS; v. 193, p. 217-228, MAR 2015.
• Citações Web of Science: 3

Resumo

Magnetic carriers are an effective option to withdraw selected target molecules from complex mixtures or to immobilize enzymes. This paper describes the synthesis of robust silica magnetic microparticles (SMMps), particularly designed for applications in bioprocesses. SMMps were synthesized in a micro-emulsion, using sodium silicate as the silica source and superparamagnetic iron oxide nanoparticles as the magnetic core. Thermally resistant particles, with high and accessible surface area, narrow particle size distribution, high saturation magnetization, and with superparamagnetic properties were obtained. Several reaction conditions were tested, yielding materials with saturation magnetization between 45 and 63 emu g(-1), particle size between 2 and 200 mu m and average diameter between 11.2 and 15.9 mu m, surface area between 49 and 103 m(2) g(-1) and pore diameter between 2 and 60 nm. The performance of SMMps in a bioprocess was evaluated by the immobilization of Pseudomonas fluorescens lipase on to octyl modified SMMp, the biocatalyst obtained was used in the production of butyl butyrate with good results. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/24277-9 - Produção de ésteres de interesse industrial em meio não-convencional catalisada por lipases imobilizadas em suportes magnéticos
• Beneficiário: Lionete Nunes de Lima
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/56572-4 - Síntese e ativação superficial de novos suportes magnéticos para imobilização de enzimas
• Beneficiário: Willian Kopp
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto