Use of experimental design to optimize a triple-potential waveform to develop a method for the determination of streptomycin and dihydrostreptomycin in pharmaceutical veterinary dosage forms by HPLC-PAD

Autor(es)

Martinez-Mejia, Monica J. ^[1] ; Rath, Susanne ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, UNICAMP, BR-13084971 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmaceutical and Biomedical Analysis; v. 104, p. 81-89, FEB 10 2015.
• Citações Web of Science: 8

Resumo

An HPLC-PAD method using a gold working electrode and a triple-potential waveform was developed for the simultaneous determination of streptomycin and dihydrostreptomycin in veterinary drugs. Glucose was used as the internal standard, and the triple-potential waveform was optimized using a factorial and a central composite design. The optimum potentials were as follows: amperometric detection, E-1= -0.15 V; cleaning potential, E-2 = +0.85 V; and reactivation of the electrode surface, E-3 = -0.65 V. For the separation of the aminoglycosides and the internal standard of glucose, a CarboPac (TM) PA1 anion exchange column was used together with a mobile phase consisting of a 0.070 mol L-1 sodium hydroxide solution in the isocratic elution mode with a flow rate of 0.8 mL min(-1). The method was validated and applied to the determination of streptomycin and dihydrostreptomycin in veterinary formulations (injection, suspension and ointment) without any previous sample pretreatment, except for the ointments, for which a liquid-liquid extraction was required before HPLC-PAD analysis. The method showed adequate selectivity, with an accuracy of 98-107% and a precision of less than 3.9%. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/01767-0 - Sorção e degradação de fármacos de uso veterinário em solos
• Beneficiário: Susanne Rath
• Modalidade de apoio: Auxílio à Pesquisa - Regular