Post-column sodiation to enhance the detection of polyacetylene glycosides in LC-DAD-MS analyses: an example from Bidens gardneri (Asteraceae)

Autor(es)

Silva, Denise Brentan ^[1] ; Rodrigues, Edilene Delphino ^{[2, 3]} ; Jose da Silva, Gil Valdo ^[2] ; Lopes, Norberto Peporine ^[1] ; Rodrigues de Oliveira, Dioneia Camilo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Fed Mato Grosso do Sul, Dept Quim, BR-79070900 Campo Grande, MS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 135, p. 87-93, APR 1 2015.
• Citações Web of Science: 4

Resumo

The use of liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) in dereplication studies of medicinal plants is a common strategy, but the analyses of polyacetylenes by LC-ESIMS are little explored and require huge efforts, especially if there are low concentrations in the extracts. A post-column sodiation strategy was successfully applied to enhance the detection of polyacetylene glycosides. Their molecular formulae were proposed by HRESI, whereas the polyacetylene chromophores were determined by UV data. The use of acetic acid in the mobile phase was essential to obtain satisfactory chromatographic resolution, and only the addition of sodium chloride solution allowed good mass spectra, internal calibration and undoubtedly the molar mass determination of polyacetylenes. This new approach has allowed the identification of polyacetylene glycosides from Bidens gardneri extract, guiding the isolation procedures, and two new compounds were obtained. The structures of the isolated polyacetylenes have been confirmed by 1D and 2D NMR, HRMS. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/18031-7 - Aplicação de MALDI-MS/MS na análise da vicenina-2 e correlatos: da compreensão dos processos ionizantes à geração de imagens
• Beneficiário: Denise Brentan da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado